Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure

doi:10.1021/jp8078887

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	Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure
	Wang, Xiao-Ling 1; Zhang, Xing-Hua 1; Cao, Meiwen 2; Zheng, Hai-Zi 1; Xiao, Bin 1; Wang, Yilin 2; Li, Ming 1
刊名	JOURNAL OF PHYSICAL CHEMISTRY B
	2009-02-26
卷号	113 期号:8 页码:2328-2332
ISSN号	1520-6106
DOI	10.1021/jp8078887
英文摘要	Using a magnetic tweezers (MT) apparatus and an atomic force microscope (AFM), we studied the condensation of DNA induced by the cationic gemini surfactant hexyl-alpha,omega-bisdodecyidimethylammonium bromide (C(12)C(6)C(12)Br(2)). Stepwise condensation events were found for forces from 0.5 to 4 pN, with a decrease in DNA extension by similar to 100 nm in each condensation event. Applied stretching forces larger than 6 pN were found to be able to decondense the condensates in a similar intermittent stepwise manner, but with similar to 60 nm of DNA released in each decondensation event. These observations are consistent with AFM images that show beadlike structures on DNA. The results lead to a model in which, during condensation, independent beadlike structures are initially formed on DNA, and as the local density of the beads increases, they compact into higher-order structures while maintaining their independence. The condensation process is different from any previously reported condensation process, but it is somewhat similar to that of chromatin assembly.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000263529500014
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/66413]
专题	中国科学院化学研究所
通讯作者	Li, Ming
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Colloid & Interface Sci, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiao-Ling,Zhang, Xing-Hua,Cao, Meiwen,et al. Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(8):2328-2332.
APA	Wang, Xiao-Ling.,Zhang, Xing-Hua.,Cao, Meiwen.,Zheng, Hai-Zi.,Xiao, Bin.,...&Li, Ming.(2009).Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure.JOURNAL OF PHYSICAL CHEMISTRY B,113(8),2328-2332.
MLA	Wang, Xiao-Ling,et al."Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure".JOURNAL OF PHYSICAL CHEMISTRY B 113.8(2009):2328-2332.