CORC  > 化学研究所  > 中国科学院化学研究所
Effects of Quantum Dots in Polymerase Chain Reaction
Wang, Libing1,2; Zhu, Yingyue1; Jiang, Yuan3; Qiao, Ruirui4; Zhu, Shuifang5; Chen, Wei1; Xu, Chuanlai1
刊名JOURNAL OF PHYSICAL CHEMISTRY B
2009-05-28
卷号113期号:21页码:7637-7641
ISSN号1520-6106
DOI10.1021/jp902404y
英文摘要The effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results showed that there was no enhancement. The mechanisms underlying, these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis.
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000266296700031
内容类型期刊论文
源URL[http://ir.iccas.ac.cn/handle/121111/66057]  
专题中国科学院化学研究所
通讯作者Chen, Wei
作者单位1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
2.Hunan Import & Export Inspect & Quarantine Bur, Changsha 410000, Hunan, Peoples R China
3.Jiangsu Import & Export Inspect & Quarantine Bur, Food Lab, Nanjing 210000, Jiangsu, Peoples R China
4.Chinese Acad Sci, Inst Chem, Beijing 100081, Peoples R China
5.Chinese Acad Inspect & Quarantine, Beijing 100082, Peoples R China
推荐引用方式
GB/T 7714
Wang, Libing,Zhu, Yingyue,Jiang, Yuan,et al. Effects of Quantum Dots in Polymerase Chain Reaction[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(21):7637-7641.
APA Wang, Libing.,Zhu, Yingyue.,Jiang, Yuan.,Qiao, Ruirui.,Zhu, Shuifang.,...&Xu, Chuanlai.(2009).Effects of Quantum Dots in Polymerase Chain Reaction.JOURNAL OF PHYSICAL CHEMISTRY B,113(21),7637-7641.
MLA Wang, Libing,et al."Effects of Quantum Dots in Polymerase Chain Reaction".JOURNAL OF PHYSICAL CHEMISTRY B 113.21(2009):7637-7641.
个性服务
查看访问统计
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。


©版权所有 ©2017 CSpace - Powered by CSpace