Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR

doi:10.1016/j.bios.2009.02.015

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	Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR
	Chen, Wei 2; Jiang, Yuan 3; Ji, Baoqing 2; Zhu, Changqin 3; Liu, Liqiang 2; Peng, Chifang 2; Jin, M. Kim 2; Qiao, Ruirui 4; Jin, Zhengyu 2; Wang, Libing 2
刊名	BIOSENSORS & BIOELECTRONICS
	2009-05-15
卷号	24 期号:9 页码:2858-2863
关键词	Mqca Gold Superparamagnetic Nanoparticles Sequential Injection Analysis Rt-pcr
ISSN号	0956-5663
DOI	10.1016/j.bios.2009.02.015
英文摘要	An ultrasensitive and rapid sequential injection analysis (SIA) based on real-time PCR (SIA-rt-PCR) assay was developed by using different nanoparticles for the detection of small molecule chemicals residues. Gold (Au) nanoparticle, conjugated with goat anti-rabbit IgG and duplex strand DNA (dsDNA), was used as a substitute for chemiluminescent probes in an SIA system. By indirect competitive immunoreactions in the SIA system, the gold nanoparticles were attached to antigens which were immobilized by superparamagnetic nanoparticles (SMNPs). The dsDNA on the gold nanoparticles was dehybridized and then the single-stranded DNA (ssDNA) was collected and quantified with rt-PCR, which showed a rather low linearity range from 2.5 atto mol L(-1) (aM) to 250 femto mol L(-1) (fM) and the LOD was 1.4 aM. This method, which is rapid, automated and capable of high-throughput, was used to detect methyl-3-quinoxaline-2-carboxylic acid (MQCA) residues in real samples. The analytical results had a coefficient of variation of less than 15% and the recovery was 89-108%. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000266192900017
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/66051]
专题	中国科学院化学研究所
通讯作者	Zhu, Shuifang
作者单位	1.Chinese Acad Inspect & Quarantine, Beijing 10008, Peoples R China 2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China 3.Jiangsu Import & Export Inspect & Quarantine Bur, Food Lab, Nanjing 210000, Jiangsu, Peoples R China 4.CAS, Inst Chem, Beijing 10008, Peoples R China
推荐引用方式 GB/T 7714	Chen, Wei,Jiang, Yuan,Ji, Baoqing,et al. Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR[J]. BIOSENSORS & BIOELECTRONICS,2009,24(9):2858-2863.
APA	Chen, Wei.,Jiang, Yuan.,Ji, Baoqing.,Zhu, Changqin.,Liu, Liqiang.,...&Xua, Chuanlai.(2009).Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR.BIOSENSORS & BIOELECTRONICS,24(9),2858-2863.
MLA	Chen, Wei,et al."Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR".BIOSENSORS & BIOELECTRONICS 24.9(2009):2858-2863.