Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes

doi:10.1016/j.bios.2009.05.031

CORC > 化学研究所 > 中国科学院化学研究所

	Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes
	Peng, Chifang 1; Li, Zhuokun 1; Zhu, Yinyue 1; Chen, Wei 1; Yuan, Yuan 1; Liu, Liqiang 1; Li, Qiusheng 1; Xu, Dinghua 1; Qiao, Ruirui 2; Wang, Libing 1
刊名	BIOSENSORS & BIOELECTRONICS
	2009-08-15
卷号	24 期号:12 页码:3657-3662
关键词	Simultaneous Sensitive Determination Multiplex Chemical Residues Multicolor Quantum Dot Probes
ISSN号	0956-5663
DOI	10.1016/j.bios.2009.05.031
英文摘要	Biotinylated denatured bovine serum albumin (Bt-dBSA)-coated cadmium telluride (CdTe) quantum dot (QD) conjugates were prepared and used to develop the multiplexed fluoroimmunoassay for the simultaneous determination of five chemical residues. An immune complex was formed using avidin as the bridge to link the Bt-dBSA-QDs with the antibodies. Primarily, individual quantitative determinations of five representative chemical residues were carried out based on the different emission properties of the QDs. Five antibodies were then conjugated with the corresponding QDs to establish the indirect competition fluorescent-linked immunosorbent assay (ic-FLISA) for the simultaneous detection of five chemicals in one well of a microplate. The linear range for dexamethason (DEX) was from 0.33 mu g/kg to 10 mu g/kg, 0.28 mu g/kg to 10 mu g/kg for gentamicin (GM), 0.16 mu g/kg to 25 mu g/kg for clonazepam (UP), 0.17 mu g/kg to 10 mu g/kg for medroxyprogesterone acetate (MPA) and 0.32 mu g/kg to 25 mu g/kg for ceftiofur (CEF), respectively. The limit of detection (LOD) for the simultaneous determination of DEX, GM, CZP, MPA and CEF were as low as 0.13 mu g/kg, 0.16 mu g/kg, 0.07 mu g/kg, 0.06 mu g/kg and 0.14 mu g/kg, respectively. This detection method was used to analyze samples of pork muscle and the recoveries ranged from 61.3% to 80.3% for DEX and from 74.0% to 87.2% for MPA. Further more, good correlation between the novel ic-FLISA and traditional ELISA was demonstrated during the determination of DEX and MPA residues in real samples. The QD-based protocol described here is less time consuming than the classical method and it may be sufficiently flexible to be used in other systems for the simultaneous multicolor detection of the drugs. (C) 2009 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000269278300040
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/65991]
专题	中国科学院化学研究所
通讯作者	Jin, Zhengyu
作者单位	1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu Prov, Peoples R China 2.CAS, Inst Chem, Beijing 10008, Peoples R China
推荐引用方式 GB/T 7714	Peng, Chifang,Li, Zhuokun,Zhu, Yinyue,et al. Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes[J]. BIOSENSORS & BIOELECTRONICS,2009,24(12):3657-3662.
APA	Peng, Chifang.,Li, Zhuokun.,Zhu, Yinyue.,Chen, Wei.,Yuan, Yuan.,...&Xu, Chuanlai.(2009).Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes.BIOSENSORS & BIOELECTRONICS,24(12),3657-3662.
MLA	Peng, Chifang,et al."Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes".BIOSENSORS & BIOELECTRONICS 24.12(2009):3657-3662.