Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis

doi:10.1110/ps.083475108

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	Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis
	Deng, Wei; Cao, Aoneng; Lai, Luhua
刊名	PROTEIN SCIENCE
	2008-06-01
卷号	17 期号:6 页码:1102-1105
关键词	Amyloid Fibril Fret Classification Cross-beta Structure Elucidation
ISSN号	0961-8368
DOI	10.1110/ps.083475108
英文摘要	The recently published microcrystal structures of amyloid fibrils from small peptides greatly enhanced our understanding of the atomic-level structure of the amyloid fibril. However, only a few amyloid fibrils can form microcrystals. The dansyl-tryptophan fluorescence resonance energy transfer (FRET) pair was shown to be able to detect the inter-peptide arrangement of the Transthyretin (105-115) amyloid fibril. In this study, we combined the known microcrystal structures with the corresponding FRET efficiencies to build a model for amyloid fibril structure classification. We found that fibrils with an antiparallel structural arrangement gave the largest FRET signal, those with a parallel arrangement gave the lowest FRET signal, and those with a mixed arrangement gave a moderate FRET signal. This confirms that the amyloid fibril structure patterns can be classified based on the FRET efficiency.
语种	英语
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS记录号	WOS:000256166600015
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/65297]
专题	中国科学院化学研究所
通讯作者	Lai, Luhua
作者单位	Peking Univ, Beijing Natl Lab Mol Sci, State Ket Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Deng, Wei,Cao, Aoneng,Lai, Luhua. Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis[J]. PROTEIN SCIENCE,2008,17(6):1102-1105.
APA	Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2008).Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis.PROTEIN SCIENCE,17(6),1102-1105.
MLA	Deng, Wei,et al."Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis".PROTEIN SCIENCE 17.6(2008):1102-1105.