Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC

CORC > 化学研究所 > 中国科学院化学研究所

	Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC
	Yang Geng-Liang 1,2; Bai Li-Gai 1; Liu Hai-Yan 1; Zhao Yu 1; Li Feng-Xin 1; Cao Wei-Min 1
刊名	CHINESE JOURNAL OF CHEMISTRY
	2008-11-01
卷号	26 期号:11 页码:2063-2066
关键词	Poly(Vinyl Ester Resin) Polymeric Monoliths Hplc Separation
ISSN号	1001-604X
英文摘要	A novel cation-exchange polymeric short column based on poly(vinyl ester resin) monolith was applied to separation in our present work. The poly(vinyl ester resin) was used as the monomer as well as the crosslinking agent in the polymerization which was induced by the initiator 2-methylpropropionitrile (AIBN). 1-Hexadecanol Was used its the porogen and methanol was used to make the 1-hexadecanol resolve more easily. Morphology of the monolithic materials was Studied by scanning electron microscopy. The capabilities of separation and loading on this column in conjunction with HPLC were investigated, respectively. The effect of pH and concentration of mobile phase (buffer) on the elution of Lys was investigated. Moreover, Lys was separated from chicken egg white with high resolution in it short time in this experiment.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000261672200019
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/65009]
专题	中国科学院化学研究所
通讯作者	Yang Geng-Liang
作者单位	1.Hebei Univ, Coll Pharm, Baoding 071002, Hebei, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Yang Geng-Liang,Bai Li-Gai,Liu Hai-Yan,et al. Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC[J]. CHINESE JOURNAL OF CHEMISTRY,2008,26(11):2063-2066.
APA	Yang Geng-Liang,Bai Li-Gai,Liu Hai-Yan,Zhao Yu,Li Feng-Xin,&Cao Wei-Min.(2008).Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC.CHINESE JOURNAL OF CHEMISTRY,26(11),2063-2066.
MLA	Yang Geng-Liang,et al."Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC".CHINESE JOURNAL OF CHEMISTRY 26.11(2008):2063-2066.