Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography Fourier transform ion cyclotron mass Spectrometry combined with O-18-Labeling

doi:10.1016/j.jasms.2007.12.002

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	Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography Fourier transform ion cyclotron mass Spectrometry combined with O-18-Labeling
	Wang, Fuyi 2,3; Weidt, Stefan 2; Xu, Jingjing 2; Mackay, C. Logan 2; Langridge-Smith, Pat R. R.2; Sadler, Peter J.1,2
刊名	JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
	2008-04-01
卷号	19 期号:4 页码:544-549
ISSN号	1044-0305
DOI	10.1016/j.jasms.2007.12.002
英文摘要	Reactions of the anticancer complex [(eta(6) -bip)Ru(en)Cl](+) (where bip is biphenyl and en is ethylenediamine) with the tripeptide glutathione (gamma-L-GIU-L-Cys-Gly; GSH), the abundant intracellular thiol, in aqueous solution give rise to two ruthenium cluster complexes, which could not be identified by electrospray mass spectrometry (ESI-MS) using a quadrupole mass analyzer. Here we use Fourier transform ion cyclotron mass spectrometry (nanoLC-FT-TCR MS) to identify the clusters separated by nanoscale liquid chromatography as the tetranuclear complex [ {eta(6) -bip)Ru(GSO(2))}(4)](2-) (2) and dinuclear complex [{eta(6) -bip)Ru(GSO(2))(2)}(2)](8-) (3) containing glutathione sulfinate (GSO(2)) ligands. Use of (OH2)-O-18 showed that oxygen from water can readily be incorporated into the oxidized glutathione ligands. These data illustrate the power of high-resolution MS for identifying highly charged multinuclear complexes and elucidating novel reaction pathways for metallodrugs, including ligand-based redox reactions.
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000255091500009
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/64449]
专题	中国科学院化学研究所
通讯作者	Sadler, Peter J.
作者单位	1.Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England 2.Univ Edinburgh, Sch Chem, Edinburgh, Midlothian, Scotland 3.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fuyi,Weidt, Stefan,Xu, Jingjing,et al. Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography Fourier transform ion cyclotron mass Spectrometry combined with O-18-Labeling[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2008,19(4):544-549.
APA	Wang, Fuyi,Weidt, Stefan,Xu, Jingjing,Mackay, C. Logan,Langridge-Smith, Pat R. R.,&Sadler, Peter J..(2008).Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography Fourier transform ion cyclotron mass Spectrometry combined with O-18-Labeling.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,19(4),544-549.
MLA	Wang, Fuyi,et al."Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography Fourier transform ion cyclotron mass Spectrometry combined with O-18-Labeling".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 19.4(2008):544-549.