Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis

doi:10.1021/ac800816k

CORC > 化学研究所 > 中国科学院化学研究所

	Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis
	Zhou, Yu 1; Shen, Hailin 2; Yi, Tie 3; Wen, Dawei 1; Pang, Nannan 1; Liao, Jie 4; Liu, Huwei 1
刊名	ANALYTICAL CHEMISTRY
	2008-12-01
卷号	80 期号:23 页码:8920-8929
ISSN号	0003-2700
DOI	10.1021/ac800816k
英文摘要	We designed and fabricated a novel microdevice to facilitate continuous adsorption phenomena for biological sample preparation. Using the device, we also developed an online, highly integrated, multifunctional strategy, with a promise of accepting a large volume of crude tissue extracts with the end point generation of a reliable MS identification within 20 min. Under an external electric field, charged membranes can adsorb multiple layers of proteins, which exceed the capacity limit of common resins or membranes. It enlarges sample loading and trapping efficiency, thus bypasses the tradeoff between sample capacity and downstream detection sensitivity. This integrated approach, formed by synergistic utilization among electric field, membrane, and fluidic handling at the microscale, reduces the overall complexity of crude samples in one step for direct MS analysis. The sample preparation goals, including enrichment, desalting, removal of noncharged contaminants, and initial fractionation, can be rapidly performed in a single device. The strategy facilitates reproducible MS quantification by circumventing traditional laborious and time-consuming sample preparation steps. In addition, MEPD extended the ion trap linear dynamic range from 2 to at least 4 orders of magnitude by eliminating ion suppression effect, enriching target analyte(s), and decreasing sample loss during integrated sample preparation.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000261335600012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/63567]
专题	中国科学院化学研究所
通讯作者	Liu, Huwei
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem,Beijing Natl Lab Mol Sci, Minist Educ,Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China 2.Univ Manchester, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England 3.Cent S Univ, Coll Informat Sci & Engn, Changsha 410083, Peoples R China 4.Gen Hosp Chinese PLA, Med Expt & Anal Ctr, Beijing 100853, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yu,Shen, Hailin,Yi, Tie,et al. Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis[J]. ANALYTICAL CHEMISTRY,2008,80(23):8920-8929.
APA	Zhou, Yu.,Shen, Hailin.,Yi, Tie.,Wen, Dawei.,Pang, Nannan.,...&Liu, Huwei.(2008).Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis.ANALYTICAL CHEMISTRY,80(23),8920-8929.
MLA	Zhou, Yu,et al."Synergistic Design of Electric Field and Membrane in Facilitating Continuous Adsorption for Cleanup and Enrichment of Proteins in Direct ESI-MS Analysis".ANALYTICAL CHEMISTRY 80.23(2008):8920-8929.