Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study

doi:10.1016/j.yexcr.2007.08.001

CORC > 化学研究所 > 中国科学院化学研究所

	Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study
	Yang, Huayan ; Yu, Junping ; Fu, Guo ; Shi, Xiaoli ; Xiao, Lin ; Chen, Yizhang ; Fang, Xiaohong ; He, Cheng
刊名	EXPERIMENTAL CELL RESEARCH
	2007-10-01
卷号	313 期号:16 页码:3497-3504
关键词	Mac-1 Icam-1 Afm Single Molecule Dynamic Force Spectroscopy Binding Probability Rupture Force Activation Living Cell
ISSN号	0014-4827
DOI	10.1016/j.yexcr.2007.08.001
英文摘要	The interaction between integrin macrophage differentiation antigen associated with complement three receptor function (Mac-1) and intercellular adhesion molecule-1 (ICAM-1), which is controlled tightly by the ligand-binding activity of Mac-1, is central to the regulation of neutrophil adhesion in host defense. Several "inside-out" signals and extracellular metal ions or antibodies have been found to activate Mac-1, resulting in an increased adhesiveness of Mac-1 to its ligands. However, the molecular basis for Mac-1 activation is not well understood yet. In this work, we have carried out a single-molecule study of Mac-1/ICAM-1 interaction force in living cells by atomic force microscopy (AFM). Our results showed that the binding probability and adhesion force of Mac-1 with ICAM-1 increased upon Mac-1 activation. Moreover, by comparing the dynamic force spectra of different Mac-1 mutants, we expected that Mac-1 activation is governed by the downward movement of its alpha 7 helix. (c) 2007 Elsevier Inc. All rights reserved.
语种	英语
出版者	ELSEVIER INC
WOS记录号	WOS:000249797100011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/62073]
专题	中国科学院化学研究所
通讯作者	Fang, Xiaohong
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Second Mil Med Univ, Inst Neurosci, Dept Neurobiol, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, State Key Lab High Field Laser Phys, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Yang, Huayan,Yu, Junping,Fu, Guo,et al. Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study[J]. EXPERIMENTAL CELL RESEARCH,2007,313(16):3497-3504.
APA	Yang, Huayan.,Yu, Junping.,Fu, Guo.,Shi, Xiaoli.,Xiao, Lin.,...&He, Cheng.(2007).Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study.EXPERIMENTAL CELL RESEARCH,313(16),3497-3504.
MLA	Yang, Huayan,et al."Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study".EXPERIMENTAL CELL RESEARCH 313.16(2007):3497-3504.