Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis

doi:10.1007/s00216-007-1167-5

CORC > 化学研究所 > 中国科学院化学研究所

	Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis
	Zhang, Li-Wei; Ding, Li; Zhang, Xin-Xiang
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2007-04-01
卷号	387 期号:8 页码:2833-2841
关键词	Affinity Capillary Electrophoresis Binding Constant Retention Factor Migration Time
ISSN号	1618-2642
DOI	10.1007/s00216-007-1167-5
英文摘要	An estimation method for determination of binding constants of receptors to ligands by affinity capillary electrophoresis was evaluated. On the basis of the theories of pseudostationary phase or so-called dynamic stationary phase, the retention factor (k) was used to represent the interaction between the receptor and ligand. k could be easily deduced from the migration times of the ligand and the receptor. Then, with the linear relationship of k versus the concentration of ligand in the running buffer, the binding constant K (b) was calculated from the slope and intercept. In order to test its feasibility, the calculation method was demonstrated using three model systems: the interactions between vancomycin and N-acetyl-Type=D-Ala-D-Ala, ristocetin and N-acetyl-D-Ala-D-Ala, and carbonic anhydrase B and an arylsulfonamide. Estimated binding constants were compared with those determined by other techniques. The results showed that this estimation method was reliable. This calculation method offers a simple and easy approach to estimating binding constants of ligands to receptors.
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000245123600027
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/61963]
专题	中国科学院化学研究所
通讯作者	Zhang, Xin-Xiang
作者单位	Peking Univ, Coll Chem, BNLMS, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Li-Wei,Ding, Li,Zhang, Xin-Xiang. Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2007,387(8):2833-2841.
APA	Zhang, Li-Wei,Ding, Li,&Zhang, Xin-Xiang.(2007).Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,387(8),2833-2841.
MLA	Zhang, Li-Wei,et al."Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 387.8(2007):2833-2841.