On-column labeling technique and chiral CE of amino acids with mixed chiral selectors and UV detection

doi:10.1002/elps.200700015

CORC > 化学研究所 > 中国科学院化学研究所

	On-column labeling technique and chiral CE of amino acids with mixed chiral selectors and UV detection
	Han, Yanli ; Chen, Yi
刊名	ELECTROPHORESIS
	2007-08-01
卷号	28 期号:15 页码:2765-2770
关键词	Amino Acid Enantiomers Chiral Ce Determination On-column Labeling Serum
ISSN号	0173-0835
DOI	10.1002/elps.200700015
英文摘要	A novel method has been developed for the on-column labeling of amino acid enantiomers with 9-fluoroenylmethyl chloroformate (FMOC), followed by chiral CE with a binary chiral selector system and UV detection. Efficient labeling was achieved by sequential injection of amino acids, borate buffer, and FMOC labeling solution at 0.2 psi for 6 s. After injection, the sandwich sections were electrically mixed at 250 V/cm. for 6 s and allowed to react (electric field-free) at room temperature for 2 min. With this procedure, successful online-labeling and chiral CE separation of 19 pairs of amino acids (AA) have been conducted, giving 17 pairs fully enantioresolved (R-s = 1.73-5.79) and two pairs partially resolved (Ala, R-s = 0.39 and Arg, R-s = 1.15) using a running buffer of 150 mM borate containing 30 mM P-CD, 30 mM sodium taurodeoxycholate (STDC), and 15% isopropanol (IPA) at pH 9.0. Chiral CE of some mixed pairs was also demonstrated, much the same as using precolumn labeling. Surprisingly, Met, Asp, Asn, Gln, and His gained even higher enantioresolution (up to 2.5%) compared with the case of precolumn labeling. As validated by both artificially prepared solutions and serum samples, the method was applicable to the quantitative determination of AA, with LODs down to 4.0 mu M. The method allowed the determination of D-AA at the ratio of 1:100 (D:L).
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000248938400026
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/61935]
专题	中国科学院化学研究所
通讯作者	Chen, Yi
作者单位	1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Han, Yanli,Chen, Yi. On-column labeling technique and chiral CE of amino acids with mixed chiral selectors and UV detection[J]. ELECTROPHORESIS,2007,28(15):2765-2770.
APA	Han, Yanli,&Chen, Yi.(2007).On-column labeling technique and chiral CE of amino acids with mixed chiral selectors and UV detection.ELECTROPHORESIS,28(15),2765-2770.
MLA	Han, Yanli,et al."On-column labeling technique and chiral CE of amino acids with mixed chiral selectors and UV detection".ELECTROPHORESIS 28.15(2007):2765-2770.