Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B

doi:10.1039/b618678e

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	Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B
	Song, Liming ; Xie, Jie ; Zhang, Chunxi ; Li, Cong ; Zhao, Jingquan
刊名	PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
	2007-06-01
卷号	6 期号:6 页码:683-688
ISSN号	1474-905X
DOI	10.1039/b618678e
英文摘要	In this work, the spectral responses of hypocrellin B (HB) to the microenvironments of various biomolecules were studied, with human serum albumin (HSA), bovine serum albumin (BSA) and ovalbumin (OVA) used as the models for proteins, sodium alginate (SOA) and hyaluronan (HYA) for polysaccharides and liposomes for lipid membranes. Generally, compared to those in aqueous solution, the absorbance and fluorescence of FIB were all strengthened in the model systems except for the fluorescence in HYA. Specially, according to the spectral responses of HB, the microenvironments in biomolecules and liposomes could be set in a sequence of hydrophobic grades, i.e., liposomes > proteins > polysaccharides. Further, R-F/A, a parameter defined as the ratio of the fluorescence intensity to the absorbance, was proposed to identify the microenvironment quantitatively. It was found that the R-F/A could not only distinguish various types of biomolecules but also identify specific binding from nonspecific binding to proteins or polysaccharides.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000247783100023
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/61635]
专题	中国科学院化学研究所
通讯作者	Zhao, Jingquan
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Yunnan Univ, Sch Engn & Chem Sci, Ctr Adv Studies Med & Organ Chem, Kunming 650091, Peoples R China
推荐引用方式 GB/T 7714	Song, Liming,Xie, Jie,Zhang, Chunxi,et al. Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B[J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,2007,6(6):683-688.
APA	Song, Liming,Xie, Jie,Zhang, Chunxi,Li, Cong,&Zhao, Jingquan.(2007).Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B.PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,6(6),683-688.
MLA	Song, Liming,et al."Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B".PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 6.6(2007):683-688.