Radiolabeling and biodistribution of a nasopharyngeal carcinoma-targeting peptide identified by in vivo phage display

doi:10.1111/j.1745-7270.2007.00321.x

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	Radiolabeling and biodistribution of a nasopharyngeal carcinoma-targeting peptide identified by in vivo phage display
	Sun, Liyan; Chu, Taiwei; Wang, Yi; Wang, Xiangyun
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2007-08-01
卷号	39 期号:8 页码:624-632
关键词	In Vivo Phage Display Biodistribution Radiolabeling Nasopharyngeal Carcinoma Deiodination
ISSN号	1672-9145
DOI	10.1111/j.1745-7270.2007.00321.x
英文摘要	A dodecapeptide EDIKPKTSLAFR ligand targeting CEN-1 human nasopharyngeal carcinoma (NPC) was identified by in vivo phage display. Two tridecapeptides and their derivatives, named YR13 (YEDIKPKTSLAFR), EY13 (EDIKPKTSLAFRY), EY13-NH2 (EDIKPKTSLAFRY-NH2) and Fmoc-YR13 (Fmoc-YEDIKPKTSLAFR), were synthesized and radiolabeled with I-131. The stability in vitro, biodistribution and tissue distribution of selected phage particles in mice bearing NPC tumor were determined, and plasma metabolites analysis of radiolabeled peptides was carried out. Although Fmoc and NH2 groups could protect the peptide from deiodination, only Fmoc group inhibited the binding of Fmoc-YR13 to NPC tumors. The compound EY13-NH2, the C-terminal amide of peptide EY13, had the greatest serum stability, the least deiodination, and showed favorable tumor/blood ratios. The selected phage particles (phage 3 or phage 5) were more concentrated in NPC tumors than the control phage (initial phage display peptide library). EY13 could also inhibit the binding of selected phage particles to tumors. The results indicated that EDIKPKTSLAFR was a good candidate in diagnostic and therapeutic NPC.
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000249205600010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/60595]
专题	中国科学院化学研究所
通讯作者	Chu, Taiwei
作者单位	Peking Univ, Coll Chem & Mol Engn, Dept Appl Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Sun, Liyan,Chu, Taiwei,Wang, Yi,et al. Radiolabeling and biodistribution of a nasopharyngeal carcinoma-targeting peptide identified by in vivo phage display[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(8):624-632.
APA	Sun, Liyan,Chu, Taiwei,Wang, Yi,&Wang, Xiangyun.(2007).Radiolabeling and biodistribution of a nasopharyngeal carcinoma-targeting peptide identified by in vivo phage display.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(8),624-632.
MLA	Sun, Liyan,et al."Radiolabeling and biodistribution of a nasopharyngeal carcinoma-targeting peptide identified by in vivo phage display".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.8(2007):624-632.