Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance

doi:10.1016/j.bbrc.2007.08.059

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	Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
	Deng, Wei; Cao, Aoneng; Lai, Luhua
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2007-10-26
卷号	362 期号:3 页码:689-694
关键词	Amyloid Fibril Formation Amyloid Fibril Structure Transthyretin (105-115) Fret Parallel P-sheet Electron Microscope
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2007.08.059
英文摘要	Transthyretin (TTR) is an amyloidogenic protein involved in many mental diseases. The peptide derived from TTR (105-115) has been widely studied as a model peptide for understanding the mechanism of amyloid fibril formation. However, the detailed arrangement of this peptide in amyloid fibril is still unclear. We have studied the amyloid fibril formation process of TTR (105-115) by introducing a pair of FRET probes into the peptide with a dansyl group at the N-terminal and a tryptophan residue at the C-terminal. Our experiment demonstrated that the strands of TTR (105-115) in the same beta-sheet may be parallel and the mating sheets may be anti-parallel to each other in the amyloid fibril. The kinetics followed by FRET and EM indicated for a possible intermediate state and the distance between sheets became shorter when the intermediate amyloid fibril turns into a more matured form. (c) 2007 Elsevier Inc. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000249602700025
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/60423]
专题	中国科学院化学研究所
通讯作者	Cao, Aoneng
作者单位	Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Deng, Wei,Cao, Aoneng,Lai, Luhua. Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,362(3):689-694.
APA	Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2007).Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,362(3),689-694.
MLA	Deng, Wei,et al."Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 362.3(2007):689-694.