Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE

doi:10.1002/elps.200700281

CORC > 化学研究所 > 中国科学院化学研究所

	Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE
	Qi, Li; Liu, Meirong; Guo, Zhenpeng; Xie, Mingyi; Qiu, Changgui; Chen, Yi
刊名	ELECTROPHORESIS
	2007-11-01
卷号	28 期号:22 页码:4150-4155
关键词	Amino Acid Enantioseparation Ligand-exchange Ce Rice-wine Product Zinc Complex
ISSN号	0173-0835
DOI	10.1002/elps.200700281
英文摘要	The aim of this work was to assay seasoning D- or L-aromatic amino acids (AAs) in rice-brewed suspensions, Laozao in Chinese, by chiral ligand-exchange CE with UV detection and Zn(II) complex as a chiral selecting system. Resolution and peak retention were found to be parallel to the basicity of the AA chiral ligands, and basic L-Arg was known to work the best at pH 8.20 compared with L-Lys and other AA ligands. Baseline separation Of DL-aromatic AAs and partially separation of some FMOC-labeled nonaromatic AAs have been achieved using a running buffer of 5 mM ammonium acetate, 100 mM boric acid, 3 mM ZnSO4, and 6 MM L-Arg at pH 8.20. The aromatic amino acids in four brands of Laozao were measured in a range of 0.25-20 mu g/mL for Typ, 1.00-120 mu g/mL for Phe, and 2.50-200 mu g/ml, for Tyr, with linear regression coefficient all over 0.999. The LOD (S/N = 3) was 0.15 mu g/mL for Typ, 0.50 mu g/mL for Phe, and 1.25 mu g/mL for Tyr. The recovery of the method determined by spiking with the supernates of Laozao as background was 94.0-112.9%. The RSDs of migration time and peak area measured from six injections of tyrosine were 0.2 and 2.7%, respectively, for run-to-run, and 1.6 and 3.2%, respectively for day-to-day. Interestingly, there were only L-Trp, D-Tyr, and L-Tyr found in the assayed four brands of Laozao. They may serye as an index to recognize the brand of Laozao.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000251363800016
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/60375]
专题	中国科学院化学研究所
通讯作者	Chen, Yi
作者单位	Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Qi, Li,Liu, Meirong,Guo, Zhenpeng,et al. Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE[J]. ELECTROPHORESIS,2007,28(22):4150-4155.
APA	Qi, Li,Liu, Meirong,Guo, Zhenpeng,Xie, Mingyi,Qiu, Changgui,&Chen, Yi.(2007).Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE.ELECTROPHORESIS,28(22),4150-4155.
MLA	Qi, Li,et al."Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE".ELECTROPHORESIS 28.22(2007):4150-4155.