An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase

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	An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase
	Li Huaqing ; Guo Zonghui ; Liu Chunxiu ; Jiang Liying ; Cui Dafu ; Liu Wenyong ; Huang Yong ; Cai Xinxia
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2006-09-01
卷号	34 页码:S1-S4
ISSN号	0253-3820
英文摘要	A two-electrode configuration has been fabricated by depositing gold film on a polyethylene terephthalate (PET) substrate. The integrated reference electrode was obtained by screen-printing commercial silver and silver chloride paste on the gold electrode. The working electrode was modified by drop-coating a redox polymer formed of poly (4-vinylpyrodine) complexed with osmium (2, 2-bipyrridine)(2). The bienzyme, horseradish peroxidase (HRP and glutamate oxidase (GLOx), were immobilized in the redox matrix by the (poly(ethylene glycol) diglycidyl ether(PEGDGE) crosslinker. The optimized biosensor were characterized by a sensitivity of 37.5 mA(mol/L)(-1) cm(-2), a detection limit of 1.0 x 10(-6) mol/L, a response time of less than 16 s, and a correlation coefficient during a concentration ranged from 1.0 x 10(-6) mol/L to 1.0 x 10(-4) mol/L is 0.9998 (n = 9). The biosensor exhibits excellent reproducibility and stability, retaining more than 90% of its original activity over a period of one month. The simple fabrication of the biosensor can be used to determinate L-glutamate rapidly in food industry and clinical diagnose.
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000244539600001
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/59865]
专题	中国科学院化学研究所
通讯作者	Cai Xinxia
作者单位	1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Chem, Joint Lab Polymer Sci & Mat, State Key Lab Polymer Phys & Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Li Huaqing,Guo Zonghui,Liu Chunxiu,et al. An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34:S1-S4.
APA	Li Huaqing.,Guo Zonghui.,Liu Chunxiu.,Jiang Liying.,Cui Dafu.,...&Cai Xinxia.(2006).An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34,S1-S4.
MLA	Li Huaqing,et al."An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34(2006):S1-S4.