Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured pply(L-lactide)

doi:10.1016/j.biomaterials.2004.11.016

CORC > 化学研究所 > 中国科学院化学研究所

	Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured pply(L-lactide)
	Wan, YQ ; Wang, Y ; Liu, ZM ; Qu, X ; Han, BX ; Bei, JZ ; Wang, SG
刊名	BIOMATERIALS
	2005-07-01
卷号	26 期号:21 页码:4453-4459
关键词	Poly(L-lactide) Polystyrene Cell Adhesion Topography Structure Osteoblast Micro-scale Nano-scale
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2004.11.016
英文摘要	The impact of the surface topography of polylactone-type polymer on cell adhesion was to be concerned because the micro-scale texture of a surface can provide a significant effect on the adhesion behavior of cells on the surface. Especially for the application of tissue engineering scaffold, the pore size could have an influence on cell in-growth and subsequent proliferation. Micro-fabrication technology was used to generate specific topography to investigate the relationship between the cells and surface. In this study the pits-patterned surfaces of polystyrene (PS) film with diameters 2.2 and 0.45 mu m were prepared by phase-separation, and the corresponding scale islands-patterned PLLA surface was prepared by a molding technique using the pits-patterned PS as a template. The adhesion and proliferation behavior of OCT-1 osteoblast-like cells morphology on the pits- and islands-patterned surface were characterized by SEM observation, cell attachment efficiency measurement and MTT assay. The results showed that the cell adhesion could be enhanced on PLLA and PS surface with nano-scale and micro-scale roughness compared to the smooth surfaces of the PLLA and PS. The OCT- 1 osteoblast-like cells could grow along the surface with two different size islands of PLLA and grow inside the micro-scale pits of the PS. However, the proliferation of cells on the micro- and nano-scale patterned surface has not been enhanced compared with the controlled smooth surface. (c) 2004 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000227579500012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/57487]
专题	中国科学院化学研究所
通讯作者	Wang, SG
作者单位	1.Chinese Acad Sci, State Key Lab Polymer Phys & Chem, Inst Chem, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Lab Collids, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Wan, YQ,Wang, Y,Liu, ZM,et al. Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured pply(L-lactide)[J]. BIOMATERIALS,2005,26(21):4453-4459.
APA	Wan, YQ.,Wang, Y.,Liu, ZM.,Qu, X.,Han, BX.,...&Wang, SG.(2005).Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured pply(L-lactide).BIOMATERIALS,26(21),4453-4459.
MLA	Wan, YQ,et al."Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured pply(L-lactide)".BIOMATERIALS 26.21(2005):4453-4459.