From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [Ru-II(bipy)(terpy)L](2+) complexes (bipy=2,2 '-bipyridine; terpy=2,2 ': 6,2 ''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c) | |
Yang, XJ; Drepper, F; Wu, B; Sun, WH; Haehnel, W; Janiak, C | |
刊名 | DALTON TRANSACTIONS |
2005 | |
期号 | 2页码:256-267 |
ISSN号 | 1477-9226 |
DOI | 10.1039/b414999h |
英文摘要 | Compounds [Ru-II(bipy)(terpy)L](PF6)(2) with bipy = 2,2'-bipyridine, terpy = 2,2':6',2"-terpyridine, L = H2O, imidazole (imi), 4-methylimidazole, 2-methylimidazole, benzimidazole, 4,5-diphenylimidazole, indazole, pyrazole, 3-methylpyrazole have been synthesized and characterized by H-1 NMR, ESI-MS and UV/Vis (in CH3CN and H2O). For L = H2O, imidazole, 4,5-diphenylimidazole and indazole the X-ray structures of the complexes have been determined with the crystal packing featuring only few intermolecular C-H...pi or pi-pi interactions due to the separating action of the PF6-anions. Complexes with L = imidazole and 4-methylimidazole exhibit a fluorescence emission with a maximum at 662 and 667 nm, respectively (lambda(exo) = 475 nm, solvent CH3CN or H2O). The substitution of the aqua ligand in [Ru(bipy)(terpy)(H2O)](2+) in aqueous solution by imidazole to give [Ru(bipy)(terpy)(imi)](2+) is fastest at a pH of 8.5 (as followed by the increase in emission intensity). Coupling of the [Ru(bipy)(terpy)](2+) fragment to cytochrome c (Yeast iso-1) starting from the Ru-aqua complex was successful at 35 degreesC and pH 7.0 after 5 d under argon in the dark. The [Ru(bipy)(terpy)(cyt c)]-product was characterized by UV/Vis, emission and mass spectrometry. The location where the [Ru(bipy)(terpy)] complex was coupled to the protein was identified as His44 (corresponding to His39 in other numbering schemes) using digestion of the Ru-coupled protein by trypsin and analysis of the tryptic peptides by HPLC-high resolution MS. |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000225889400006 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/57161] |
专题 | 中国科学院化学研究所 |
通讯作者 | Janiak, C |
作者单位 | 1.Univ Freiburg, Inst Anorgan & Analyt Chem, D-79104 Freiburg, Germany 2.Chinese Acad Sci, Inst Chem, CAS Key Lab Engn Plast, Beijing 100080, Peoples R China 3.Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany |
推荐引用方式 GB/T 7714 | Yang, XJ,Drepper, F,Wu, B,et al. From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [Ru-II(bipy)(terpy)L](2+) complexes (bipy=2,2 '-bipyridine; terpy=2,2 ': 6,2 ''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c)[J]. DALTON TRANSACTIONS,2005(2):256-267. |
APA | Yang, XJ,Drepper, F,Wu, B,Sun, WH,Haehnel, W,&Janiak, C.(2005).From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [Ru-II(bipy)(terpy)L](2+) complexes (bipy=2,2 '-bipyridine; terpy=2,2 ': 6,2 ''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c).DALTON TRANSACTIONS(2),256-267. |
MLA | Yang, XJ,et al."From model compounds to protein binding: syntheses, characterizations and fluorescence studies of [Ru-II(bipy)(terpy)L](2+) complexes (bipy=2,2 '-bipyridine; terpy=2,2 ': 6,2 ''-terpyridine; L = imidazole, pyrazole and derivatives, cytochrome c)".DALTON TRANSACTIONS .2(2005):256-267. |
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