Real-time SPR phase detection for protein micro-array

doi:10.3866/PKU.WHXB20050813

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	Real-time SPR phase detection for protein micro-array
	Yu, XL ; Wei, X ; Wang, DX ; Ding, X ; Liao, W ; Zhao, XS
刊名	ACTA PHYSICO-CHIMICA SINICA
	2005-08-01
卷号	21 期号:8 页码:888-892
关键词	Protein Micro-array Spr Phase Detection Interference Of Polarized Light
ISSN号	1000-6818
DOI	10.3866/PKU.WHXB20050813
英文摘要	Combining surface plasmon resonance (SPR) and spatial phase modulation, a real-time SPR sensing chip was illuminated with a collimated parallel light beam. A Wollaston prism was introduced into the detection light path, causing the p- and s-polarization components to interfere with each other. The information of biochemical reactions could be obtained by extracting the phase change from the interference patterns. The sensor chips, on which the Au film was 30 mn and 40 nm, experimented with 50% and 20% alcohol solutions respectively to obtain SPR array graph. Subsequently, two proteins, rabbit IgG as positive sample and Bovine Serum Albumin (BSA) as negative control, reacted with the mixed solution of 0.2 mL goat-anti-rabbit IgG sample and 1.8 mL deionized water, using Au film of 30 nm. The reaction diagram was also attained. The experimental results indicated that, with the merits of high noise resistance, high sensitivity, unlabelled and real-time detection, this SPR method could meet the requirement of protein micro-array detection.
语种	英语
出版者	PEKING UNIV PRESS
WOS记录号	WOS:000231293900014
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/56857]
专题	中国科学院化学研究所
通讯作者	Yu, XL
作者单位	1.Tsinghua Univ, Dept Precis Instruments & Mechanol, State Key Lab Precis Measurement Technol & Instru, Beijing 100084, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Yu, XL,Wei, X,Wang, DX,et al. Real-time SPR phase detection for protein micro-array[J]. ACTA PHYSICO-CHIMICA SINICA,2005,21(8):888-892.
APA	Yu, XL,Wei, X,Wang, DX,Ding, X,Liao, W,&Zhao, XS.(2005).Real-time SPR phase detection for protein micro-array.ACTA PHYSICO-CHIMICA SINICA,21(8),888-892.
MLA	Yu, XL,et al."Real-time SPR phase detection for protein micro-array".ACTA PHYSICO-CHIMICA SINICA 21.8(2005):888-892.