Aptamer-based ATP assay using a luminescent light switching complex

doi:10.1021/ac050165w

CORC > 化学研究所 > 中国科学院化学研究所

	Aptamer-based ATP assay using a luminescent light switching complex
	Wang, J ; Jiang, YX ; Zhou, CS ; Fang, XH
刊名	ANALYTICAL CHEMISTRY
	2005-06-01
卷号	77 期号:11 页码:3542-3546
ISSN号	0003-2700
DOI	10.1021/ac050165w
英文摘要	With the increasing applications of nucleic acid aptamers as a new class of molecular recognition probes in bioanalysis and biosensor development, the development of general and simple signaling strategies to transduce aptamer-target binding events to detectable signals is demanding. We have developed a new signaling method based on aptamers and a DNA molecular light switching complex, [Ru(phen)(2)(dppz)](2+), for sensitive protein detection. In this work, we have demonstrated the applicability of this signaling mechanism to small-molecule detection using ATP as a model target. Our results have shown that upon ATP binding to the folded aptamer where [Ru(phen)(2)(dppz)](2+) intercalated, the conformational change or distortion of the aptamer is large enough to cause a significant luminescence change of [Ru(phen)2(dppz)](2+). By monitoring the ATP-dependent luminescence intensity change, we have achieved ATP detection with high selectivity and high sensitivity down to 1 nM in homogeneous solution. The method is very simple without the needs for covalently labeling aptamers or using costly enzymes and multistep analysis as other reported fluorescence/luminescence assays for ATP. The successful detection of ATP indicates that using the signaling aptamers with [Ru(phen)(2)(dppz)](2+) is expected to be a general method for aptamer-based target detection.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000229561800026
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/56285]
专题	中国科学院化学研究所
通讯作者	Fang, XH
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 2.Sichuan Univ, Coll Chem, Chengdu 610044, Peoples R China
推荐引用方式 GB/T 7714	Wang, J,Jiang, YX,Zhou, CS,et al. Aptamer-based ATP assay using a luminescent light switching complex[J]. ANALYTICAL CHEMISTRY,2005,77(11):3542-3546.
APA	Wang, J,Jiang, YX,Zhou, CS,&Fang, XH.(2005).Aptamer-based ATP assay using a luminescent light switching complex.ANALYTICAL CHEMISTRY,77(11),3542-3546.
MLA	Wang, J,et al."Aptamer-based ATP assay using a luminescent light switching complex".ANALYTICAL CHEMISTRY 77.11(2005):3542-3546.