Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species

doi:10.1016/j.abb.2005.02.028

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	Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species
	Shi, HL ; Timmins, G ; Monske, M ; Burdick, A ; Kalyanaraman, B ; Liu, Y ; Clement, JL ; Burchiel, S ; Liu, KJ
刊名	ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
	2005-05-01
卷号	437 期号:1 页码:59-68
关键词	Bmpo Dmpo Depmpo Deppepo Spin Trap Superoxide Radical Anion Epr Cell
ISSN号	0003-9861
DOI	10.1016/j.abb.2005.02.028
英文摘要	Electron paramagnetic resonance with spin trapping is a useful technique to detect reactive oxygen species, such as superoxide radical anion (O-2(center dot-)), a key species in many biological processes. We evaluated the abilities of four spin traps in trapping cell-generated O-2(center dot-): 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BMPO), 2-diethoxyphosphoryl-2-phenethyl-3,4-dihydro-2H-pyrrole N-oxide (DEPPEPO), 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Optimal experimental conditions for obtaining maximal signal intensity of O-2(center dot-) adduct in a cellular system were first studied. The maximal intensities of BMPO, DEPMPO, and DMPO adducts were similar while DEPPEPO did not trap cell-generated O-2(center dot-) induced by 1,6-benzo[a]pyrene quinone in a human mammary epithelial cell line (MCF-10A). BMPO and DEPMPO adducts were more stable, considering the stability of their maximal signal, than DMPO adduct in the tested cellular systems. In addition, we observed that O-2(center dot-) spin adducts were reduced to their corresponding hydroxyl adducts in the cellular system. The selection of optimal spin trap in trapping cell-generated O-2(center dot-) is discussed. (c) 2005 Elsevier Inc. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000228513900007
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/56223]
专题	中国科学院化学研究所
通讯作者	Liu, KJ
作者单位	1.Univ New Mexico, Coll Pharm, Albuquerque, NM 87131 USA 2.Med Coll Wisconsin, Biophys Res Inst, Milwaukee, WI 53226 USA 3.Med Coll Wisconsin, Free Rad Res Ctr, Milwaukee, WI 53226 USA 4.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 5.CNRS, UMR 6517, Lab SREP, F-13397 Marseille, France 6.Univ Aix Marseille 1, Case 521, F-13397 Marseille, France 7.Univ Aix Marseille 3, Case 521, F-13397 Marseille, France
推荐引用方式 GB/T 7714	Shi, HL,Timmins, G,Monske, M,et al. Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2005,437(1):59-68.
APA	Shi, HL.,Timmins, G.,Monske, M.,Burdick, A.,Kalyanaraman, B.,...&Liu, KJ.(2005).Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,437(1),59-68.
MLA	Shi, HL,et al."Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 437.1(2005):59-68.