Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2)

doi:10.1016/j.bbrc.2013.10.092

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	Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2)
	Qiu, Shunchen 1; Lai, Luhua 1,2
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2013-11-15
卷号	441 期号:2 页码:453-456
关键词	Human Non-pancreatic Secretory Phospholipase a(2) Bactericidal Activity Gram-positive Bacteria Gram-negative Bacteria Ph
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2013.10.092
英文摘要	Human non-pancreatic secretory phospholipase A(2) (hnpsPLA(2)) is a group IIA phospholipase A(2) which plays an important role in the innate immune response. This enzyme was found to exhibit bactericidal activity toward Gram-positive bacteria, but not Gram-negative ones. Though native hnpsPLA(2) is active over a broad pH range, it is only highly active at alkaline conditions with the optimum activity pH of about 8.5. In order to make it highly active at neutral pH, we have obtained two hnpsPLA(2) mutants, Glu89Lys and Arg100Glu that work better at neutral pH in a previous study. In the present study, we tested the bactericidal effects of the native hnpsPLA(2) and the two mutants. Both native hnpsPLA(2) and the two mutants exhibit bactericidal activity toward Gram-positive bacteria. Furthermore, they can also kill Escherichia coil, a Gram-negative bacterium. The two mutants showed better bactericidal activity for E. coil at neutral pH than the native enzyme, which is consistent with the enzyme activities. As hnpsPLA(2) is highly stable and biocompatible, it may provide a promising therapy for bacteria infection treatment or other bactericidal applications. (C) 2013 Elsevier Inc. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000327290100030
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/54115]
专题	中国科学院化学研究所
通讯作者	Lai, Luhua
作者单位	1.Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, BNLMS, Beijing 100871, Peoples R China 2.Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Qiu, Shunchen,Lai, Luhua. Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,441(2):453-456.
APA	Qiu, Shunchen,&Lai, Luhua.(2013).Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,441(2),453-456.
MLA	Qiu, Shunchen,et al."Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 441.2(2013):453-456.