Polystyrene Spheres-Assisted Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Quantitative Analysis of Plasma Lysophosphatidylcholines

doi:10.1021/ac400452k

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	Polystyrene Spheres-Assisted Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Quantitative Analysis of Plasma Lysophosphatidylcholines
	Wei, Yanbo 1,2; Li, Shumu 3; Wang, Jingxia 3; Shu, Chunying 3; Liu, Jian'an 2; Xiong, Shaoxiang 2; Song, Jianwen 4; Zhang, Junjie 4; Zhao, Zhenwen 1,2
刊名	ANALYTICAL CHEMISTRY
	2013-05-07
卷号	85 期号:9 页码:4729-4734
ISSN号	0003-2700
DOI	10.1021/ac400452k
英文摘要	The quantitative analysis by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) is a challenge due to the poor reproducibility originating from the heterogeneity of the matrix-analyte crystals. Polystyrene (PS) colloidal spheres have superior monodispersed property and can self-assemble to form photonic crystals. With the assistance of PS spheres, a uniform matrix-analyte cocrystal was constructed for the quantitative analysis of plasma lysophosphatidylcholines (LPCs). The matrix and the solvent in MALDI MS analysis were optimized, and the reproducibility and the accuracy were investigated in detail. This is the first report about the very simple method of PS spheres assisted MALDI MS for quantitative analysis, where it is believed that this approach will greatly expand the applications of MALDI MS.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000318756100073
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/54059]
专题	中国科学院化学研究所
通讯作者	Zhao, Zhenwen
作者单位	1.Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Beijing Mass Spectrum Ctr, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 4.Beijing Normal Univ, Coll Life Sci, Key Lab Cell Proliferat & Regulat Biol, Minist Educ,Inst Cell Biol, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Wei, Yanbo,Li, Shumu,Wang, Jingxia,et al. Polystyrene Spheres-Assisted Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Quantitative Analysis of Plasma Lysophosphatidylcholines[J]. ANALYTICAL CHEMISTRY,2013,85(9):4729-4734.
APA	Wei, Yanbo.,Li, Shumu.,Wang, Jingxia.,Shu, Chunying.,Liu, Jian'an.,...&Zhao, Zhenwen.(2013).Polystyrene Spheres-Assisted Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Quantitative Analysis of Plasma Lysophosphatidylcholines.ANALYTICAL CHEMISTRY,85(9),4729-4734.
MLA	Wei, Yanbo,et al."Polystyrene Spheres-Assisted Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Quantitative Analysis of Plasma Lysophosphatidylcholines".ANALYTICAL CHEMISTRY 85.9(2013):4729-4734.