Photodynamic efficacy of liposome-delivered hypocrellin B in microvascular endothelial cells in vitro and chicken combs in vivo: a potential photosensitizer for port wine stain

doi:10.1088/1054-660X/23/2/025605

CORC > 化学研究所 > 中国科学院化学研究所

	Photodynamic efficacy of liposome-delivered hypocrellin B in microvascular endothelial cells in vitro and chicken combs in vivo: a potential photosensitizer for port wine stain
	Chen, H. X.1,2; Yang, Z. F.3; Zou, X. B.1; Zhu, J. G.2; Deng, H.4; Zhao, J. Q.4; Gu, Y.2
刊名	LASER PHYSICS
	2013-02-01
卷号	23 期号:2
ISSN号	1054-660X
DOI	10.1088/1054-660X/23/2/025605
英文摘要	Photodynamic therapy (PDT) has been proved a successful method for port wine stain (PWS), but the prolonged skin photosensitivity induced by the photosensitizers used currently seriously limits the clinical application of PDT. In this study, we investigate the feasibility of hypocrellin B (HB), a promising second-generation photosensitizer for the treatment of PWS. The photodynamic effect of liposome-delivered HB was evaluated in vitro with microvascular endothelial cells (MEC) and in vivo with chicken combs. The dark cytotoxicity and photocytotoxicity of liposomal HB in MEC were evaluated using the MTT assay. Gross and histological examinations were performed to investigate the selective occlusion of the superficial dermal microvasculature in the chicken comb. The result showed that photocytotoxicity of liposomal HB was dependent on both light dose and drug concentration. PDT with HB (0.5-1 mg kg(-1)) and a light dose of 120 J cm(-2) showed selective destruction of the superficial dermal microvasculature of the chicken comb, leaving the overlying epidermis intact. This is the first study to investigate the potential efficacy of HB-PDT as a novel modality for the treatment of PWS. These findings suggest that liposomal HB is a safe and effective photosensitizer for PWS.
语种	英语
出版者	IOP PUBLISHING LTD
WOS记录号	WOS:000318005200025
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/53583]
专题	中国科学院化学研究所
通讯作者	Chen, H. X.
作者单位	1.Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Dept Dermatol, Beijing, Peoples R China 2.Chinese Peoples Liberat Army Gen Hosp, Dept Laser Med, Beijing, Peoples R China 3.Beijing Inst Radiat Med, Lab Electromagnetism & Laser Biol, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Chen, H. X.,Yang, Z. F.,Zou, X. B.,et al. Photodynamic efficacy of liposome-delivered hypocrellin B in microvascular endothelial cells in vitro and chicken combs in vivo: a potential photosensitizer for port wine stain[J]. LASER PHYSICS,2013,23(2).
APA	Chen, H. X..,Yang, Z. F..,Zou, X. B..,Zhu, J. G..,Deng, H..,...&Gu, Y..(2013).Photodynamic efficacy of liposome-delivered hypocrellin B in microvascular endothelial cells in vitro and chicken combs in vivo: a potential photosensitizer for port wine stain.LASER PHYSICS,23(2).
MLA	Chen, H. X.,et al."Photodynamic efficacy of liposome-delivered hypocrellin B in microvascular endothelial cells in vitro and chicken combs in vivo: a potential photosensitizer for port wine stain".LASER PHYSICS 23.2(2013).