Ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry for accurate analysis of glycerophospholipids and sphingolipids in drug resistance tumor cells

doi:10.1016/j.chroma.2015.01.013

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	Ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry for accurate analysis of glycerophospholipids and sphingolipids in drug resistance tumor cells
	Li, Lin 1,2; Wang, Linlin 1; Shangguan, Dihua 1; Wei, Yanbo 1,2; Han, Juanjuan 1; Xiong, Shaoxiang 1; Zhao, Zhenwen 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2015-02-13
卷号	1381 页码:140-148
关键词	Uhplc-esi-ms/ms Glycerophospholipids Sphingolipids Mcf-7 Mcf-7r
ISSN号	0021-9673
DOI	10.1016/j.chroma.2015.01.013
英文摘要	Glycerophospholipids and sphingolipids are important signaling molecules which are involved in many physiological and pathological processes. Here we reported an effective method for accurate analysis of these lipids by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The methanol method was adopted for extraction of lipids due to its simplicity and high efficiency. It was found that two subclasses of sphingolipids, sulfatide (ST) and cerebroside (CB), were heat labile, so a decreased temperature in the ion source of MS might be necessary for these compounds analysis. In addition, it was found that the isobaric interferences were commonly existent, for example, the m/z of 16:0/18:1 PC containing two C-13 isotope being identical to that of 16:0/18:0 PC determined by a unit mass resolution mass spectrometer; therefore, a baseline separation of interferential species was required to maintain selectivity and accuracy of analysis. In this work, an ultra-high-performance liquid chromatography (UHPLC)-based method was developed for separation of interferential species. Moreover, in order to deal with the characteristics of different polarity and wide dynamic range of glycerophospholipids and sphingolipids in biological systems, three detecting conditions were combined together for comprehensive and rational analysis of glycerophospholipids and sphingolipids. The method was utilized to profile glycerophospholipids and sphingolipids in drug resistant tumor cells. Our results showed that many lipids were significantly changed in drug resistant tumor cells compared to paired drug sensitive tumor cells. This is a systematic report about the isobaric interferences and heat labile compounds interferences when analyzing glycerophospholipids and sphingolipids by ESI-MS/MS, which aids in ruling out one potential source of systematic error to ensure the accuracy of analysis. (C) 2015 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000349590600016
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/53375]
专题	中国科学院化学研究所
通讯作者	Zhao, Zhenwen
作者单位	1.Chinese Acad Sci, Beijing Mass Spectrum Ctr, Inst Chem, Beijing Natl Lab Mol Sci,Key Lab Analyt Chem Livi, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Lin,Wang, Linlin,Shangguan, Dihua,et al. Ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry for accurate analysis of glycerophospholipids and sphingolipids in drug resistance tumor cells[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1381:140-148.
APA	Li, Lin.,Wang, Linlin.,Shangguan, Dihua.,Wei, Yanbo.,Han, Juanjuan.,...&Zhao, Zhenwen.(2015).Ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry for accurate analysis of glycerophospholipids and sphingolipids in drug resistance tumor cells.JOURNAL OF CHROMATOGRAPHY A,1381,140-148.
MLA	Li, Lin,et al."Ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry for accurate analysis of glycerophospholipids and sphingolipids in drug resistance tumor cells".JOURNAL OF CHROMATOGRAPHY A 1381(2015):140-148.