A Cresyl Violet-Based Fluorescent Off-On Probe for the Detection and Imaging of Hypoxia and Nitroreductase in Living Organisms

doi:10.1002/asia.201402364

CORC > 化学研究所 > 中国科学院化学研究所

	A Cresyl Violet-Based Fluorescent Off-On Probe for the Detection and Imaging of Hypoxia and Nitroreductase in Living Organisms
	Wan, Qiong-Qiong; Gao, Xing-Hui; He, Xin-Yuan; Chen, Su-Ming; Song, Yan-Chao; Gong, Qiu-Yu; Li, Xiao-Hua; Ma, Hui-Min
刊名	CHEMISTRY-AN ASIAN JOURNAL
	2014-08-01
卷号	9 期号:8 页码:2058-2062
关键词	Amines Fluorescence Imaging Fluorescent Probe Hypoxic Tumor Cells Reduction
ISSN号	1861-4728
DOI	10.1002/asia.201402364
英文摘要	A new cresyl violet-based fluorescent off-on probe has been developed through a one-step synthesis for the detection of nitroreductase (NTR) and hypoxia. The detection mechanism is based on the NTR-catalyzed reduction of the probe to cresyl violet, accompanied with a large fluorescence enhancement at a long wavelength of 625 nm. The probe can detect NTR in aqueous solution with high selectivity and sensitivity, and the detection limit is 1 ngmL(-1) NTR. Most importantly, the probe has been successfully used to image not only NTR and hypoxia in living cells, but also the distribution of NTR in zebrafish in vivo.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000342675900010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/52957]
专题	中国科学院化学研究所
通讯作者	Ma, Hui-Min
作者单位	Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wan, Qiong-Qiong,Gao, Xing-Hui,He, Xin-Yuan,et al. A Cresyl Violet-Based Fluorescent Off-On Probe for the Detection and Imaging of Hypoxia and Nitroreductase in Living Organisms[J]. CHEMISTRY-AN ASIAN JOURNAL,2014,9(8):2058-2062.
APA	Wan, Qiong-Qiong.,Gao, Xing-Hui.,He, Xin-Yuan.,Chen, Su-Ming.,Song, Yan-Chao.,...&Ma, Hui-Min.(2014).A Cresyl Violet-Based Fluorescent Off-On Probe for the Detection and Imaging of Hypoxia and Nitroreductase in Living Organisms.CHEMISTRY-AN ASIAN JOURNAL,9(8),2058-2062.
MLA	Wan, Qiong-Qiong,et al."A Cresyl Violet-Based Fluorescent Off-On Probe for the Detection and Imaging of Hypoxia and Nitroreductase in Living Organisms".CHEMISTRY-AN ASIAN JOURNAL 9.8(2014):2058-2062.