Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin

doi:10.1038/srep05395

CORC > 化学研究所 > 中国科学院化学研究所

	Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
	Yuan, Yuan 1; Jiang, Chao 3; Liu, Libing 2; Yu, Shulin 1; Cui, Zhanhu 1; Chen, Min 1; Lin, Shufang 1; Wang, Shu 2; Huang, Luqi 1
刊名	SCIENTIFIC REPORTS
	2014-06-23
卷号	4
ISSN号	2045-2322
DOI	10.1038/srep05395
英文摘要	In this work, a rapid (within 4-5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products.
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000337888600009
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/52365]
专题	中国科学院化学研究所
通讯作者	Liu, Libing
作者单位	1.Chinese Acad Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Daodi Herbs, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Beijing Normal Univ, Beijing Key Lab Protect & Applicat Chinese Med Re, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Yuan,Jiang, Chao,Liu, Libing,et al. Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin[J]. SCIENTIFIC REPORTS,2014,4.
APA	Yuan, Yuan.,Jiang, Chao.,Liu, Libing.,Yu, Shulin.,Cui, Zhanhu.,...&Huang, Luqi.(2014).Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin.SCIENTIFIC REPORTS,4.
MLA	Yuan, Yuan,et al."Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin".SCIENTIFIC REPORTS 4(2014).