DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer

doi:10.1371/journal.pone.0100243

CORC > 化学研究所 > 中国科学院化学研究所

	DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer
	Wang, Yuanyuan 1; Luo, Yun 1; Bing, Tao 2; Chen, Zheng 1; Lu, Minhua 1; Zhang, Nan 2; Shangguan, Dihua 2; Gao, Xin 1
刊名	PLOS ONE
	2014-06-23
卷号	9 期号:6
ISSN号	1932-6203
DOI	10.1371/journal.pone.0100243
英文摘要	Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000338917900035
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/52099]
专题	中国科学院化学研究所
通讯作者	Shangguan, Dihua
作者单位	1.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urol, Guangzhou 510275, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yuanyuan,Luo, Yun,Bing, Tao,et al. DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer[J]. PLOS ONE,2014,9(6).
APA	Wang, Yuanyuan.,Luo, Yun.,Bing, Tao.,Chen, Zheng.,Lu, Minhua.,...&Gao, Xin.(2014).DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer.PLOS ONE,9(6).
MLA	Wang, Yuanyuan,et al."DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer".PLOS ONE 9.6(2014).