Construction of 3D Micropatterned Surfaces with Wormlike and Superhydrophilic PEG Brushes To Detect Dysfunctional Cells

doi:10.1021/am506983q

CORC > 化学研究所 > 中国科学院化学研究所

	Construction of 3D Micropatterned Surfaces with Wormlike and Superhydrophilic PEG Brushes To Detect Dysfunctional Cells
	Hou, Jianwen 1,2; Shi, Qiang 1; Ye, Wei 1,2; Fan, Qunfu 3; Shi, Hengchong 1; Wong, Shing-Chung 4; Xu, Xiaodong 3; Yin, Jinghua 1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2014-12-20
卷号	6 期号:23 页码:20868-20879
关键词	Superhydrophilicity 3d Patterned Surface Poly(Ethylene Glycol) Brushes Phosphatidylserine Dysfunctional Cell Detection
ISSN号	1944-8244
DOI	10.1021/am506983q
英文摘要	Detection of dysfunctional and apoptotic cells plays an important role in clinical diagnosis and therapy. To develop a portable and user-friendly platform for dysfunctional and aging cell detection, we present a facile method to construct 3D patterns on the surface of styrene-b-(ethylene-co-butylene)-b-styrene elastomer (SEBS) with poly(ethylene glycol) brushes. Normal red blood cells (RBCs) and lysed RBCs (dysfunctional cells) are used as model cells. The strategy is based on the fact that poly(ethylene glycol) brushes tend to interact with phosphatidylserine, which is in the inner leaflet of normal cell membranes but becomes exposed in abnormal or apoptotic cell membranes. We demonstrate that varied patterned surfaces can be obtained by selectively patterning atom transfer radical polymerization (ATRP) initiators on the SEBS surface via an aqueous-based method and growing PEG brushes through surface-initiated atom transfer radical polymerization. The relatively high initiator density and polymerization temperature facilitate formation of PEG brushes in high density, which gives brushes worm-like morphology and superhydrophilic property; the tendency of dysfunctional cells adhered on the patterned surfaces is completely different from well-defined arrays of normal cells on the patterned surfaces, providing a facile method to detect dysfunctional cells effectively. The PEG-patterned surfaces are also applicable to detect apoptotic HeLa cells. The simplicity and easy handling of the described technique shows the potential application in microdiagnostic devices.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000346326600037
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/51917]
专题	中国科学院化学研究所
通讯作者	Shi, Qiang
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Harbin Engn Univ, Coll Mat Sci & Chem Engn, Polymer Mat Res Ctr, Harbin 150001, Peoples R China 4.Univ Akron, Dept Mech Engn, Akron, OH 44325 USA
推荐引用方式 GB/T 7714	Hou, Jianwen,Shi, Qiang,Ye, Wei,et al. Construction of 3D Micropatterned Surfaces with Wormlike and Superhydrophilic PEG Brushes To Detect Dysfunctional Cells[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(23):20868-20879.
APA	Hou, Jianwen.,Shi, Qiang.,Ye, Wei.,Fan, Qunfu.,Shi, Hengchong.,...&Yin, Jinghua.(2014).Construction of 3D Micropatterned Surfaces with Wormlike and Superhydrophilic PEG Brushes To Detect Dysfunctional Cells.ACS APPLIED MATERIALS & INTERFACES,6(23),20868-20879.
MLA	Hou, Jianwen,et al."Construction of 3D Micropatterned Surfaces with Wormlike and Superhydrophilic PEG Brushes To Detect Dysfunctional Cells".ACS APPLIED MATERIALS & INTERFACES 6.23(2014):20868-20879.