Inhibition of Hepatitis C Virus Infection by DNA Aptamer against NS2 Protein

doi:10.1371/journal.pone.0090333

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	Inhibition of Hepatitis C Virus Infection by DNA Aptamer against NS2 Protein
	Gao, Yimin 1; Yu, Xiaoyan 1; Xue, Binbin 1; Zhou, Fei 1; Wang, Xiaohong 1; Yang, Darong 1,2; Liu, Nianli 1,2; Xu, Li 3; Fang, Xiaohong 3; Zhu, Haizhen 1,2
刊名	PLOS ONE
	2014-02-28
卷号	9 期号:2
ISSN号	1932-6203
DOI	10.1371/journal.pone.0090333
英文摘要	NS2 protein is essential for hepatitis C virus (HCV) replication. NS2 protein was expressed and purified. Aptamers against NS2 protein were raised and antiviral effects of the aptamers were examined. The molecular mechanism through which the aptamers exert their anti-HCV activity was investigated. The data showed that aptamer NS2-3 inhibited HCV RNA replication in replicon cell line and infectious HCV cell culture system. NS2-3 and another aptamer NS2-2 were demonstrated to inhibit infectious virus production without cytotoxicity in vitro. They did not affect hepatitis B virus replication. Interferon beta (IFN-beta) and interferon-stimulated genes (ISGs) were not induced by the aptamers in HCV-infected hepatocytes. Furthermore, our study showed that N-terminal region of NS2 protein is involved in the inhibition of HCV infection by NS2-2. I861T within NS2 is the major resistance mutation identified. Aptamer NS2-2 disrupts the interaction of NS2 with NS5A protein. The data suggest that NS2-2 aptamer against NS2 protein exerts its antiviral effects through binding to the N-terminal of NS2 and disrupting the interaction of NS2 with NS5A protein. NS2-specific aptamer is the first NS2 inhibitor and can be used to understand the mechanisms of virus replication and assembly. It may be served as attractive candidates for inclusion in the future HCV direct-acting antiviral combination therapies.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000332396200173
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50897]
专题	中国科学院化学研究所
通讯作者	Zhu, Haizhen
作者单位	1.Hunan Univ, Dept Mol Med, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China 2.Cent S Univ, Xiangya Med Sch, Res Ctr Canc Prevent & Treatment & Translat Med, Res Ctr Liver Canc,Hunan Prov Tumor Hosp,Affiliat, Changsha, Hunan, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yimin,Yu, Xiaoyan,Xue, Binbin,et al. Inhibition of Hepatitis C Virus Infection by DNA Aptamer against NS2 Protein[J]. PLOS ONE,2014,9(2).
APA	Gao, Yimin.,Yu, Xiaoyan.,Xue, Binbin.,Zhou, Fei.,Wang, Xiaohong.,...&Zhu, Haizhen.(2014).Inhibition of Hepatitis C Virus Infection by DNA Aptamer against NS2 Protein.PLOS ONE,9(2).
MLA	Gao, Yimin,et al."Inhibition of Hepatitis C Virus Infection by DNA Aptamer against NS2 Protein".PLOS ONE 9.2(2014).