Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase

doi:10.1007/s10337-014-2691-z

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	Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase
	Chen, Tengfei 1,2,3; Gu, Jiangyong 2; Wang, Hao 2; Yuan, Gu 2; Chen, Lirong 2; Xu, Xiaojie 2; Xiao, Wei 1,3
刊名	CHROMATOGRAPHIA
	2014-07-01
卷号	77 期号:13-14 页码:893-899
关键词	Liquid Chromatography Molecularly Imprinted Polymer Semi-preparative Separation Emodin Rheum PaL.atum L.
ISSN号	0009-5893
DOI	10.1007/s10337-014-2691-z
英文摘要	In this work, a core-shell molecularly imprinted polymer (MIP) was synthesized through sol-gel coating procedure by using silica beads, emodin, 3-aminopropyltriethoxysilane, tetraethoxysilane and tetrahydrofuran as supporting matrix, template, functional monomer, cross-linker and solvent, respectively. The selective recognition property for emodin and its analogues (physcion and aloe emodin) of the resultant MIP was evaluated in a chromatographic column for high-performance liquid chromatography (HPLC). The retention time and imprinted factor of MIP column for emodin, physcion and aloe emodin were 5.11, 0.63, 0.69 min and 4.69, 0.75, 1.38, respectively. It showed that the MIP beads had a good binding ability for emodin. Finally, one-step separation of emodin from alcohol extract of Rheum palmatum L. at semi-preparative scale was achieved and 380 mg of emodin was collected in 5 days. The product was characterized by mass spectrometry and HPLC and its purity was 95 %.
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000339005800004
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50761]
专题	中国科学院化学研究所
通讯作者	Xiao, Wei
作者单位	1.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Rare Earth Mat Chem & Applicat, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Jiangsu Kanion Pharmaceut Co Ltd, State Key Lab New Tech Chinese Med Pharmaceut Pro, Lianyungang 222001, Peoples R China
推荐引用方式 GB/T 7714	Chen, Tengfei,Gu, Jiangyong,Wang, Hao,et al. Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase[J]. CHROMATOGRAPHIA,2014,77(13-14):893-899.
APA	Chen, Tengfei.,Gu, Jiangyong.,Wang, Hao.,Yuan, Gu.,Chen, Lirong.,...&Xiao, Wei.(2014).Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase.CHROMATOGRAPHIA,77(13-14),893-899.
MLA	Chen, Tengfei,et al."Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase".CHROMATOGRAPHIA 77.13-14(2014):893-899.