Inhibition of hepatitis C virus infection by NS5A-specific aptamer

doi:10.1016/j.antiviral.2014.03.020

CORC > 化学研究所 > 中国科学院化学研究所

	Inhibition of hepatitis C virus infection by NS5A-specific aptamer
	Yu, Xiaoyan 1; Gao, Yimin 1; Xue, Binbin 1; Wang, Xiaohong 1; Yang, Darong 1,2; Qin, Yuwen 1; Yu, Rong 1; Liu, Nianli 1,2; Xu, Li 3; Fang, Xiaohong 3
刊名	ANTIVIRAL RESEARCH
	2014-06-01
卷号	106 页码:116-124
关键词	Hcv Ns5a Aptamer Antivirals Replication Mutation
ISSN号	0166-3542
DOI	10.1016/j.antiviral.2014.03.020
英文摘要	To increase efficacy of hepatitis C treatment, future regiments will incorporate multiple direct-acting antiviral drugs. HCV NS5A protein was expressed and purified. Aptamers against NS5A were screened and obtained by the selective evolution of ligands by exponential enrichment approach and the antiviral actions of the aptamers were tested. The mechanisms through which the aptamers exert their antiviral activity were explored. The aptamers NS5A-4 and NS5A-5 inhibit HCV RNA replication and infectious virus production without causing cytotoxicity in human hepatocytes. The aptamers do not affect hepatitis B virus replication in HepG2.2.15 cells. Interferon beta (IFN-beta) and interferon-stimulated genes (ISGs) are not induced by the aptamers in HCV-infected hepatocytes. Further study shows that domain I and domain III of NS5A protein are involved in the suppression of HCV RNA replication and infectious virus production by NS5A-4. Y2105H within NS5A is the major resistance mutation identified. NS5A aptamer disrupts the interaction of NS5A with core protein. The data suggest that the aptamers against NS5A protein may exert antiviral effects through inhibiting viral RNA replication, preventing the interaction of NS5A with core protein. Aptamers for NS5A may be used to understand the mechanisms of virus replication and assembly and served as potential therapeutic agents for hepatitis C. (C) 2014 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000336827700015
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50123]
专题	中国科学院化学研究所
通讯作者	Zhu, Haizhen
作者单位	1.Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Dept Mol Med, Changsha 410082, Hunan, Peoples R China 2.Cent S Univ, Affiliated Tumor Hosp, Xiangya Med Sch,Res Ctr Canc Prevent & Treatment, Hunan Prov Tumor Hosp,Translat Med Res Ctr Liver, Changsha, Hunan, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Yu, Xiaoyan,Gao, Yimin,Xue, Binbin,et al. Inhibition of hepatitis C virus infection by NS5A-specific aptamer[J]. ANTIVIRAL RESEARCH,2014,106:116-124.
APA	Yu, Xiaoyan.,Gao, Yimin.,Xue, Binbin.,Wang, Xiaohong.,Yang, Darong.,...&Zhu, Haizhen.(2014).Inhibition of hepatitis C virus infection by NS5A-specific aptamer.ANTIVIRAL RESEARCH,106,116-124.
MLA	Yu, Xiaoyan,et al."Inhibition of hepatitis C virus infection by NS5A-specific aptamer".ANTIVIRAL RESEARCH 106(2014):116-124.