Bioelectrocatalytic Oxygen Reduction Reaction by Bilirubin Oxidase Adsorbed on Glassy Carbon and Edge-Plane Pyrolytic Graphite Electrodes: Effect of Redox Mediators

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	Bioelectrocatalytic Oxygen Reduction Reaction by Bilirubin Oxidase Adsorbed on Glassy Carbon and Edge-Plane Pyrolytic Graphite Electrodes: Effect of Redox Mediators
	Li, De-an 1; Okajima, Takeyoshi 1; Mao, Lanqun 2; Ohsaka, Takeo 1
刊名	INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
	2014-03-01
卷号	9 期号:3 页码:1390-1398
关键词	Bilirubin Oxidase Direct Electron Transfer Intramolecular Electron Transfer Oxygen Reduction Reaction Bioelectrocatalysis Redox Mediators
ISSN号	1452-3981
英文摘要	Direct electron transfer (DET) of bilirubin oxidase (BOx, from Myrothecium verrucaria) adsorbed on edge-plane pyrolytic graphite (EPPG) electrode as well as an electron transfer between the EPPG or glassy carbon (GC) electrode and the BOx (adsorbed on these electrodes) via a mediation by redox mediators ([Fe(CN)(6)](3-) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)), i.e., a mediator-assisted electron transfer, have been examined under anaerobic and aerobic conditions by cyclic voltammetry and differential pulse voltammetry. On differential pulse voltammograms on the EPPG electrode two redox processes with formal potentials of 0.50 and 0.29 V vs. Ag/AgCl/NaCl(3M) were clearly observed in 0.05 M phosphate buffer (pH 5.0), corresponding to the redox transformations of the T1 site and the T2/T3 cluster of the BOx, respectively. DET was not observed on the GC electrode. A mediator-assisted electron transfer between the electrode and the BOx adsorbed on it was realized in the absence and the presence of [Fe(CN)(6)](3-) and ABTS through the bioelectrocatalytic oxygen reduction reaction (ORR) by the BOx, and in addition its overall electron transfer mechanism is discussed briefly including the DET, mediator-assisted electron transfer and an intramolecular electron transfer (IET) from the T1 site to the T2/T3 cluster site, in which an uphill IET reaction during the catalytic ORR is suggested.
语种	英语
出版者	ESG
WOS记录号	WOS:000331194200027
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50097]
专题	中国科学院化学研究所
通讯作者	Li, De-an
作者单位	1.Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Elect Chem, Midori Ku, Yokohama, Kanagawa 2268502, Japan 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Li, De-an,Okajima, Takeyoshi,Mao, Lanqun,et al. Bioelectrocatalytic Oxygen Reduction Reaction by Bilirubin Oxidase Adsorbed on Glassy Carbon and Edge-Plane Pyrolytic Graphite Electrodes: Effect of Redox Mediators[J]. INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE,2014,9(3):1390-1398.
APA	Li, De-an,Okajima, Takeyoshi,Mao, Lanqun,&Ohsaka, Takeo.(2014).Bioelectrocatalytic Oxygen Reduction Reaction by Bilirubin Oxidase Adsorbed on Glassy Carbon and Edge-Plane Pyrolytic Graphite Electrodes: Effect of Redox Mediators.INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE,9(3),1390-1398.
MLA	Li, De-an,et al."Bioelectrocatalytic Oxygen Reduction Reaction by Bilirubin Oxidase Adsorbed on Glassy Carbon and Edge-Plane Pyrolytic Graphite Electrodes: Effect of Redox Mediators".INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE 9.3(2014):1390-1398.