Comparison of Cytotoxicity of Pristine and Covalently Functionalized Multi-Walled Carbon Nanotubes in RAW 264.7 Macrophages

doi:10.1166/jnn.2012.5700

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	Comparison of Cytotoxicity of Pristine and Covalently Functionalized Multi-Walled Carbon Nanotubes in RAW 264.7 Macrophages
	Luo, Man 1; Deng, Xiaoyong 2; Shen, Xizhong 1; Dong, Ling 1; Liu, Yuanfang 2,3
刊名	JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
	2012
卷号	12 期号:1 页码:274-283
关键词	Multi-walled Carbon Nanotubes Raw 264.7 Macrophage Cell Viability Apoptosis Intracellular Calcium Concentration
ISSN号	1533-4880
DOI	10.1166/jnn.2012.5700
英文摘要	Carbon nanotubes may be applied in different fields including biomedicine and mechanical engineering. It is important to understand the potential hazards of carbon nanotubes. In the present study, the toxicological effects of the pristine multi-walled carbon nanotubes (p-MWCNTs) and taurine functionalized multi-walled carbon nanotubes (tau-MWCNTs) were assessed on RAW 264.7 macrophages. We tested cell viability, GSH/GSSG ratio, apoptosis, intracellular calcium concentration, ultrastructural changes of cell morphology, and the release of IL-8. We observed the loss of cell viability, decline in the cellular GSH/GSSG ratio, increase of IL-8, and the increase of intracellular calcium concentration in RAW 264.7 macrophages when exposed to p-MWCNTs at high dosage. Additionally, exposure to p-MWCNTs resulted in ultrastructural and morphological changes in RAW 264.7 macrophages. In contrast, the RAW 264.7 macrophages exposed to the tau-MWCNTs did not exhibit altered morphology. Our results conclude that the tau-MWCNTs show lower toxicity than that of p-MWCNTs.
语种	英语
出版者	AMER SCIENTIFIC PUBLISHERS
WOS记录号	WOS:000301990500033
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/49865]
专题	中国科学院化学研究所
通讯作者	Shen, Xizhong
作者单位	1.Fudan Univ, Shanghai Med Coll, Zhongshan Hosp, Shanghai 200032, Peoples R China 2.Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Luo, Man,Deng, Xiaoyong,Shen, Xizhong,et al. Comparison of Cytotoxicity of Pristine and Covalently Functionalized Multi-Walled Carbon Nanotubes in RAW 264.7 Macrophages[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2012,12(1):274-283.
APA	Luo, Man,Deng, Xiaoyong,Shen, Xizhong,Dong, Ling,&Liu, Yuanfang.(2012).Comparison of Cytotoxicity of Pristine and Covalently Functionalized Multi-Walled Carbon Nanotubes in RAW 264.7 Macrophages.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,12(1),274-283.
MLA	Luo, Man,et al."Comparison of Cytotoxicity of Pristine and Covalently Functionalized Multi-Walled Carbon Nanotubes in RAW 264.7 Macrophages".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 12.1(2012):274-283.