Rational Design of a Ratiometric and Targetable Fluorescent Probe for Imaging Lysosomal Zinc Ions

doi:10.1021/ic301307v

CORC > 化学研究所 > 中国科学院化学研究所

	Rational Design of a Ratiometric and Targetable Fluorescent Probe for Imaging Lysosomal Zinc Ions
	Xue, Lin 1; Li, Guoping 1,2; Zhu, Dongjian 1,2; Liu, Qing 1,2; Jiang, Hua 1
刊名	INORGANIC CHEMISTRY
	2012-10-15
卷号	51 期号:20 页码:10842-10849
ISSN号	0020-1669
DOI	10.1021/ic301307v
英文摘要	Fluorescent detecting and tracking of zinc ions in living cells has become more and more important because the physiological and pathological functions of zinc are highly associated with the timing and discrete distribution of subcellular zinc ion. For the detection of subcellular zinc concentrations with high spatial and temporal reliability, we report the design, synthesis, properties, and bioimaging evaluation of a fluorescent probe, DQZn4, composed of a quinoline scaffold as the ratiometric signaling unit for Zn2+ and a dimethylethylamino group as the targeting anchor for lysosomes. In acidic aqueous solution (pH = 5.2), DQZn4 features fluorescence emission maximum at 542 nm due to the resonance charge transfer in 4-alkoxy substituted quinoline. Upon binding Zn2+, the probe displays significant fluorescent turn-on and ratiometric detection of Zn2+ with blue shift of 47 run and remarkable fluorescence ratio changes (R = F-495/F-542 (nm), R/R-0 = 5.1). Confocal imaging experiments establish that DQZn4 is able to localize to lysosomes and respond to lysosomal zinc changes in living cells by using fluorescence ratiometry.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000309739100041
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/49239]
专题	中国科学院化学研究所
通讯作者	Jiang, Hua
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, CAS Key Lab Photochem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xue, Lin,Li, Guoping,Zhu, Dongjian,et al. Rational Design of a Ratiometric and Targetable Fluorescent Probe for Imaging Lysosomal Zinc Ions[J]. INORGANIC CHEMISTRY,2012,51(20):10842-10849.
APA	Xue, Lin,Li, Guoping,Zhu, Dongjian,Liu, Qing,&Jiang, Hua.(2012).Rational Design of a Ratiometric and Targetable Fluorescent Probe for Imaging Lysosomal Zinc Ions.INORGANIC CHEMISTRY,51(20),10842-10849.
MLA	Xue, Lin,et al."Rational Design of a Ratiometric and Targetable Fluorescent Probe for Imaging Lysosomal Zinc Ions".INORGANIC CHEMISTRY 51.20(2012):10842-10849.