A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells

doi:10.1039/c2an36011j

CORC > 化学研究所 > 中国科学院化学研究所

	A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells
	Jin, Yue 1; Chen, Cheng 1; Meng, Lingchen 1; Chen, Jitao 1; Li, Meixian 1; Zhu, Zhiwei 1; Lin, Jian 2
刊名	ANALYST
	2012
卷号	137 期号:23 页码:5571-5575
ISSN号	0003-2654
DOI	10.1039/c2an36011j
英文摘要	A fast, simple and quantitative approach was established for monitoring autophagy in HeLa cells by directly detecting the conversion of green fluorescent protein (GFP) labelled autophagy markers, GFP-LC3-I to GFP-LC3-II, in crude cellular extract using capillary electrophoresis (CE) with laser-induced fluorescence (LIF). Compared with the traditional methods, this proposed method is simpler and more reliable. Moreover, high sensitivity, with a limit of detection (LOD) of 0.48 ppt (bovine serum albumin as standard protein), was obtained by an in-capillary derivatization method coupled with a field-enhanced sample injection (FESI) technique, this may allow the success of this technique in the detection of endogenous markers of autophagy in cells.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000310375200018
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/48775]
专题	中国科学院化学研究所
通讯作者	Zhu, Zhiwei
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Jin, Yue,Chen, Cheng,Meng, Lingchen,et al. A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells[J]. ANALYST,2012,137(23):5571-5575.
APA	Jin, Yue.,Chen, Cheng.,Meng, Lingchen.,Chen, Jitao.,Li, Meixian.,...&Lin, Jian.(2012).A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells.ANALYST,137(23),5571-5575.
MLA	Jin, Yue,et al."A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells".ANALYST 137.23(2012):5571-5575.