A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(epsilon-caprolactone): synthesis, self-assembly, and protein encapsulation behavior

doi:10.1039/c2py20253k

CORC > 化学研究所 > 中国科学院化学研究所

	A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(epsilon-caprolactone): synthesis, self-assembly, and protein encapsulation behavior
	Li, Bin 2,3; Chen, Gao 2,3; Meng, Fanbo 1; Li, Taihang 2,3; Yue, Jun 2,3; Jing, Xiabin 3; Huang, Yubin 3
刊名	POLYMER CHEMISTRY
	2012
卷号	3 期号:9 页码:2421-2429
ISSN号	1759-9954
DOI	10.1039/c2py20253k
英文摘要	New amphiphilic graft copolymers with hydrophilic poly(ethylene oxide-co-allyl glycidyl ether) (PEAG) as the backbone and hydrophobic poly(epsilon-caprolactone) (PCL) as the side chains (PEAG-g-PCL) were designed and synthesized. For this purpose, the combined techniques of anionic copolymerization, radical mediated thiol-ene reaction and ring-opening polymerization were employed. The properties of the graft copolymer were characterized by H-1 NMR, C-13 NMR, GPC and MALDI-TOF-MS. The self-assembly of the copolymers in aqueous solution was examined by fluorescence spectroscopy, DLS and TEM. The results showed that with a relatively low critical aggregation concentration (CAC), the graft copolymers could form different morphologies including vesicles (polymersomes). The formed polymersomes had the capacity to encapsulate protein molecules (like hemoglobin, Hb) through a modified lyophilization-rehydration method as analyzed via TEM and SDS-PAGE. Furthermore the gas-binding capacity of the encapsulated Hb was assayed via UV-vis spectroscopy. The characteristic absorption peak of the encapsulated Hb in different gas-binding states (CO, O-2, N-2) showed no significant change by comparison with that of free Hb. These Hb encapsulated PEAG-g-PCL polymersomes could have the potential to be applied as an artificial oxygen carrier for transfusion.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000306967600013
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/48017]
专题	中国科学院化学研究所
通讯作者	Meng, Fanbo
作者单位	1.Jilin Univ, China Japan Union Hosp, Dept Cardiol, Changchun 130033, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
推荐引用方式 GB/T 7714	Li, Bin,Chen, Gao,Meng, Fanbo,et al. A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(epsilon-caprolactone): synthesis, self-assembly, and protein encapsulation behavior[J]. POLYMER CHEMISTRY,2012,3(9):2421-2429.
APA	Li, Bin.,Chen, Gao.,Meng, Fanbo.,Li, Taihang.,Yue, Jun.,...&Huang, Yubin.(2012).A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(epsilon-caprolactone): synthesis, self-assembly, and protein encapsulation behavior.POLYMER CHEMISTRY,3(9),2421-2429.
MLA	Li, Bin,et al."A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(epsilon-caprolactone): synthesis, self-assembly, and protein encapsulation behavior".POLYMER CHEMISTRY 3.9(2012):2421-2429.