Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells

doi:10.1039/c2jm31582c

CORC > 化学研究所 > 中国科学院化学研究所

	Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells
	Song, Yanchao; Shi, Wen; Chen, Wei; Li, Xiaohua; Ma, Huimin
刊名	JOURNAL OF MATERIALS CHEMISTRY
	2012
卷号	22 期号:25 页码:12568-12573
ISSN号	0959-9428
DOI	10.1039/c2jm31582c
英文摘要	In this work, an efficient approach for targeting and detecting cancer cells has been developed through the design of the assembly of fluorescent carbon nanodots and folic acid (C-dots-FA), which is endocytosible by the overexpressed folate receptor (FR) molecule. The fluorescent C-dots were prepared by a facile microwave pyrolysis method, but their surfaces were passivated with 4,7,10-trioxa-1,13-tridecanediamine, so that active amino groups could be engineered for the further conjugation with FA. The uptake of the designed C-dots-FA by HeLa cancer cells, as revealed by confocal laser scanning microscopy, is via receptor-mediated endocytosis, which is further confirmed by competition experiments as well as a comparative study with FR-negative MCF-7 cells. The proposed method shows excellent biocompatibility, and, most notably, its applicability to discriminating FR-positive cancer cells from normal cells has been successfully demonstrated by culturing and analyzing the first model cell mixture of NIH-3T3 and HeLa cells, which makes it of great potential for cancer diagnosis studies.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000304884000022
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47783]
专题	中国科学院化学研究所
通讯作者	Ma, Huimin
作者单位	Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Song, Yanchao,Shi, Wen,Chen, Wei,et al. Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells[J]. JOURNAL OF MATERIALS CHEMISTRY,2012,22(25):12568-12573.
APA	Song, Yanchao,Shi, Wen,Chen, Wei,Li, Xiaohua,&Ma, Huimin.(2012).Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells.JOURNAL OF MATERIALS CHEMISTRY,22(25),12568-12573.
MLA	Song, Yanchao,et al."Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells".JOURNAL OF MATERIALS CHEMISTRY 22.25(2012):12568-12573.