Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells | |
Song, Yanchao; Shi, Wen; Chen, Wei; Li, Xiaohua; Ma, Huimin | |
刊名 | JOURNAL OF MATERIALS CHEMISTRY
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2012 | |
卷号 | 22期号:25页码:12568-12573 |
ISSN号 | 0959-9428 |
DOI | 10.1039/c2jm31582c |
英文摘要 | In this work, an efficient approach for targeting and detecting cancer cells has been developed through the design of the assembly of fluorescent carbon nanodots and folic acid (C-dots-FA), which is endocytosible by the overexpressed folate receptor (FR) molecule. The fluorescent C-dots were prepared by a facile microwave pyrolysis method, but their surfaces were passivated with 4,7,10-trioxa-1,13-tridecanediamine, so that active amino groups could be engineered for the further conjugation with FA. The uptake of the designed C-dots-FA by HeLa cancer cells, as revealed by confocal laser scanning microscopy, is via receptor-mediated endocytosis, which is further confirmed by competition experiments as well as a comparative study with FR-negative MCF-7 cells. The proposed method shows excellent biocompatibility, and, most notably, its applicability to discriminating FR-positive cancer cells from normal cells has been successfully demonstrated by culturing and analyzing the first model cell mixture of NIH-3T3 and HeLa cells, which makes it of great potential for cancer diagnosis studies. |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000304884000022 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/47783] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Ma, Huimin |
作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Song, Yanchao,Shi, Wen,Chen, Wei,et al. Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells[J]. JOURNAL OF MATERIALS CHEMISTRY,2012,22(25):12568-12573. |
APA | Song, Yanchao,Shi, Wen,Chen, Wei,Li, Xiaohua,&Ma, Huimin.(2012).Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells.JOURNAL OF MATERIALS CHEMISTRY,22(25),12568-12573. |
MLA | Song, Yanchao,et al."Fluorescent carbon nanodots conjugated with folic acid for distinguishing folate-receptor-positive cancer cells from normal cells".JOURNAL OF MATERIALS CHEMISTRY 22.25(2012):12568-12573. |
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