A Highly Emissive Conjugated Polyelectrolyte Vector for Gene Delivery and Transfection

doi:10.1002/adma.201202145

CORC > 化学研究所 > 中国科学院化学研究所

	A Highly Emissive Conjugated Polyelectrolyte Vector for Gene Delivery and Transfection
	Feng, Xuli 1; Lv, Fengting 1; Liu, Libing 1; Yang, Qiong 1; Wang, Shu 1; Bazan, Guillermo C.2,3
刊名	ADVANCED MATERIALS
	2012-10-23
卷号	24 期号:40 页码:5428-5432
关键词	Conjugated Polyelectrolyte Vector Cell Imaging Gene Delivery And Transfection
ISSN号	0935-9648
DOI	10.1002/adma.201202145
英文摘要	An intrinsically fluorescent cationic polyfluorene (CCP) has been designed, synthesized, characterized, and examined as a plasmid DNA (pDNA) delivery vector. This material facilitates nucleic acid binding, encapsulation and efficient cellular uptake. CCP can effectively protect pDNA against nuclease degradation, which is necessary for gene carriers. Green fluorescent protein (GFP) expression experiments reveal that CCP can achieve efficient delivery and transfection of pDNA encoding GFP gene with 92% efficiency, which surpasses that of commercial transfection agents, lipofectamine 2000 (Lipo) and polyethylenimine (PEI). CCP is also highly fluorescent, with 43% quantum yield in water, and exhibits excellent photostability, which allows for real-time tracking the location of gene delivery and transfection. These features and capabilities represent a major step toward designing and applying conjugated polymers that function in both imaging and therapeutic applications.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000309909600003
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47493]
专题	中国科学院化学研究所
通讯作者	Liu, Libing
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China 2.Univ Calif Santa Barbara, Dept Chem & Biochem, Ctr Polymers & Organ Solids, Santa Barbara, CA 93106 USA 3.Univ Calif Santa Barbara, Dept Mat, Ctr Polymers & Organ Solids, Santa Barbara, CA 93106 USA
推荐引用方式 GB/T 7714	Feng, Xuli,Lv, Fengting,Liu, Libing,et al. A Highly Emissive Conjugated Polyelectrolyte Vector for Gene Delivery and Transfection[J]. ADVANCED MATERIALS,2012,24(40):5428-5432.
APA	Feng, Xuli,Lv, Fengting,Liu, Libing,Yang, Qiong,Wang, Shu,&Bazan, Guillermo C..(2012).A Highly Emissive Conjugated Polyelectrolyte Vector for Gene Delivery and Transfection.ADVANCED MATERIALS,24(40),5428-5432.
MLA	Feng, Xuli,et al."A Highly Emissive Conjugated Polyelectrolyte Vector for Gene Delivery and Transfection".ADVANCED MATERIALS 24.40(2012):5428-5432.