Thin Layer Chromatography/Plasma Assisted Multiwavelength Laser Desorption Ionization Mass Spectrometry for Facile Separation and Selective Identification of Low Molecular Weight Compounds

doi:10.1021/ac202732y

CORC > 化学研究所 > 中国科学院化学研究所

	Thin Layer Chromatography/Plasma Assisted Multiwavelength Laser Desorption Ionization Mass Spectrometry for Facile Separation and Selective Identification of Low Molecular Weight Compounds
	Zhang, Jialing; Zhou, Zhigui; Yang, Jianwang; Zhang, Wei; Bai, Yu; Liu, Huwei
刊名	ANALYTICAL CHEMISTRY
	2012-02-07
卷号	84 期号:3 页码:1496-1503
ISSN号	0003-2700
DOI	10.1021/ac202732y
英文摘要	A novel plasma assisted multiwavelength (1064, 532, and 355 nm) laser desorption ionization mass spectrometry (PAMLDI-MS) system was fabricated and applied in the analysis of low molecular weight compounds through combination with thin layer chromatography (TLC). The TLC/PAMLDI-MS system successfully integrated TLC, the multiwavelength laser ablation, and the excitated state plasma from direct analysis in real time (DART) and was proved to be effective in the facile separation and selective identification of low molecular weight compounds. An automated three-dimensional platform was utilized to facilitate the analysis procedures with all the parameters of the TLC/PAMLDI-MS systematically optimized, and the desorption/ionization mechanisms were discussed. The successful combination of three-wavelength laser with DART based system extended the range of the analytes and provided broad possibilities for the compound desorption from the TLC. The experimental results clearly showed that the laser desorption was wavelength dependent. The PAMLDI-MS system was successfully applied in the detection of low molecular weight compounds from different kinds of samples separated on a normal-phase silica gel, such as dye mixtures, drug standards, and tea extract, with the detection level of 5 ng/mm(2).
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000299863200045
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47305]
专题	中国科学院化学研究所
通讯作者	Bai, Yu
作者单位	Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Beijing Natl Lab Mol, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jialing,Zhou, Zhigui,Yang, Jianwang,et al. Thin Layer Chromatography/Plasma Assisted Multiwavelength Laser Desorption Ionization Mass Spectrometry for Facile Separation and Selective Identification of Low Molecular Weight Compounds[J]. ANALYTICAL CHEMISTRY,2012,84(3):1496-1503.
APA	Zhang, Jialing,Zhou, Zhigui,Yang, Jianwang,Zhang, Wei,Bai, Yu,&Liu, Huwei.(2012).Thin Layer Chromatography/Plasma Assisted Multiwavelength Laser Desorption Ionization Mass Spectrometry for Facile Separation and Selective Identification of Low Molecular Weight Compounds.ANALYTICAL CHEMISTRY,84(3),1496-1503.
MLA	Zhang, Jialing,et al."Thin Layer Chromatography/Plasma Assisted Multiwavelength Laser Desorption Ionization Mass Spectrometry for Facile Separation and Selective Identification of Low Molecular Weight Compounds".ANALYTICAL CHEMISTRY 84.3(2012):1496-1503.