Fabrication of PP-g-PEGMA-g-heparin and its hemocompatibility: From protein adsorption to anticoagulant tendency

doi:10.1016/j.apsusc.2012.02.113

CORC > 化学研究所 > 中国科学院化学研究所

	Fabrication of PP-g-PEGMA-g-heparin and its hemocompatibility: From protein adsorption to anticoagulant tendency
	Jin, Jing 1,2; Jiang, Wei 1; Shi, Qiang 1; Zhao, Jie 1,2; Yin, Jinghua 1; Stagnaro, Paola 3
刊名	APPLIED SURFACE SCIENCE
	2012-05-15
卷号	258 期号:15 页码:5841-5849
关键词	Hemocompatibility Heparin Peg Polypropylene Protein Adsorption
ISSN号	0169-4332
DOI	10.1016/j.apsusc.2012.02.113
英文摘要	We described a two-step process to fabricate the heparinized polypropylene (PP) film using cyanuric chloride (CC) as a trifunctional reagent and poly (ethylene glycol) methacrylate (PEGMA) as a spacer. The modified PP films were characterized by attenuated total reflectance FT-IR and X-ray photoelectron spectroscopy; the content of PEGMA and heparin were determined by gravimetric method and a toluidine blue assay, respectively. For the PP-g-PEGMA films, it was found that small size protein BSA tended to adsorb on the surface of low molecular weight monomer grafted PP, whereas big spindle-shaped fibrinogen tended to adsorb on the surface of high molecular weight monomer grafted PP. We gave a definition of anti-protein adsorptive factor r with two model proteins, albumin and fibrinogen. The results by platelet adhesion and plasma recalcification time (PRT) experiments indicated that the factor r could be used to quantitatively evaluate the anticoagulant tendency of PP-g-PEGMA modified films. For the PP-g-PEGMA-g-heparin modified films, the surface was proved to have a high bioactivity by the adsorption of AT III assay and very low platelet adhesion. It indicated that immobilization of heparin on the PP film with PEGMA as a spacer was an effective way to improve the hemocompatibility of PP. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000302135700052
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47303]
专题	中国科学院化学研究所
通讯作者	Jiang, Wei
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.CNR, Ist Studio Macromol, I-16149 Genoa, Italy
推荐引用方式 GB/T 7714	Jin, Jing,Jiang, Wei,Shi, Qiang,et al. Fabrication of PP-g-PEGMA-g-heparin and its hemocompatibility: From protein adsorption to anticoagulant tendency[J]. APPLIED SURFACE SCIENCE,2012,258(15):5841-5849.
APA	Jin, Jing,Jiang, Wei,Shi, Qiang,Zhao, Jie,Yin, Jinghua,&Stagnaro, Paola.(2012).Fabrication of PP-g-PEGMA-g-heparin and its hemocompatibility: From protein adsorption to anticoagulant tendency.APPLIED SURFACE SCIENCE,258(15),5841-5849.
MLA	Jin, Jing,et al."Fabrication of PP-g-PEGMA-g-heparin and its hemocompatibility: From protein adsorption to anticoagulant tendency".APPLIED SURFACE SCIENCE 258.15(2012):5841-5849.