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	Single-molecule monitoring in living cells by use of fluorescence microscopy
	Luo, Wangxi; He, Kangmin; Xia, Tie; Fang, Xiaohong
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2013
卷号	405期号:1页码:43-49
关键词	Single-molecule Imaging Fluorescence Microscopy Tirfm Living Cell
ISSN号	1618-2642
DOI	10.1007/s00216-012-6373-0
英文摘要	Monitoring single molecules in living cells is becoming a powerful tool for study of the location, dynamics, and kinetics of individual biomolecules in real time. In recent decades, several optical imaging techniques, for example epi-fluorescence microscopy, total internal reflection fluorescence microscopy (TIRFM), confocal microscopy, quasi-TIRFM, and single-point edge excitation subdiffraction microscopy (SPEED), have been developed, and their capability of capturing single-molecule dynamics in living cells has been demonstrated. In this review, we briefly summarize recent advances in the use of these imaging techniques for monitoring single-molecules in living cells for a better understanding of important biological processes, and discuss future developments.
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000313064000010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/46593]
专题	中国科学院化学研究所
通讯作者	Fang, Xiaohong
作者单位	Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Luo, Wangxi,He, Kangmin,Xia, Tie,et al. Single-molecule monitoring in living cells by use of fluorescence microscopy[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(1):43-49.
APA	Luo, Wangxi,He, Kangmin,Xia, Tie,&Fang, Xiaohong.(2013).Single-molecule monitoring in living cells by use of fluorescence microscopy.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(1),43-49.
MLA	Luo, Wangxi,et al."Single-molecule monitoring in living cells by use of fluorescence microscopy".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.1(2013):43-49.

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