A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C-60-carboxyfullerenes | |
Li, Ruimin1; Zhen, Mingming1; Guan, Mirong1; Chen, Daiqin1; Zhang, Guoqiang1; Ge, Jiechao2; Gong, Ping3; Wang, Chunru1; Shu, Chunying1 | |
刊名 | BIOSENSORS & BIOELECTRONICS |
2013-09-15 | |
卷号 | 47页码:502-507 |
关键词 | C-60-carboxyfullerenes Peroxidase-like Activity Colorimetric Sensor Glucose |
ISSN号 | 0956-5663 |
DOI | 10.1016/j.bios.2013.03.057 |
英文摘要 | C-60-carboxyfullerene, C-60[C(COOH)(2)](2), has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction. Kinetic studies further indicate that C-60[C(COOH)(2)](2) has an even higher affinity to TMB than that of the natural enzyme, horseradish peroxidase (HRP). C-60[C(COOH)(2)](2)/glucose oxidase (GO(x))/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 1.0-40 mu M with a limit of detection 0.5 mu M (3 sigma/slope). Moreover, this sensitive and selective sensor can be successfully applied for the quantitative determination of glucose in human serum. The results indicate that it is a simple, cheap, convenient, highly selective, sensitive, and easy handling colorimetric assay. (C) 2013 Elsevier B.V. All rights reserved. |
语种 | 英语 |
出版者 | ELSEVIER ADVANCED TECHNOLOGY |
WOS记录号 | WOS:000320481900076 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/46563] |
专题 | 中国科学院化学研究所 |
通讯作者 | Ge, Jiechao |
作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100190, Peoples R China 3.Peking Univ, Hosp 3, Beijing 100191, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Ruimin,Zhen, Mingming,Guan, Mirong,et al. A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C-60-carboxyfullerenes[J]. BIOSENSORS & BIOELECTRONICS,2013,47:502-507. |
APA | Li, Ruimin.,Zhen, Mingming.,Guan, Mirong.,Chen, Daiqin.,Zhang, Guoqiang.,...&Shu, Chunying.(2013).A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C-60-carboxyfullerenes.BIOSENSORS & BIOELECTRONICS,47,502-507. |
MLA | Li, Ruimin,et al."A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C-60-carboxyfullerenes".BIOSENSORS & BIOELECTRONICS 47(2013):502-507. |
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