Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane

doi:10.1016/j.febslet.2013.10.041

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	Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane
	Yang, Yong 1; Wolfram, Joy 1,2; Shen, Jianliang 1; Zhao, Yuliang 2,3; Fang, Xiaohong 4; Shen, Haifa 1,5; Ferrari, Mauro 1,6
刊名	FEBS LETTERS
	2013-12-11
卷号	587 期号:24 页码:3912-3920
关键词	Smad4 Membrane Docking Single-molecule Fluorescence Imaging Clathrin Caveolin-1
ISSN号	0014-5793
DOI	10.1016/j.febslet.2013.10.041
英文摘要	Transforming growth factor beta (TGF-beta) signaling is important for many biological processes. Although the sequential events of this cascade are known, the dynamics remain speculative. Here, live-cell single-molecule total internal reflection fluorescence microscopy was used to monitor the dynamics of SMAD4, a TGF-beta downstream effector, in MDA-MB-231 breast cancer cells. Contrary to previous belief, SMAD4 was detectable at the cytoplasmic membrane, displaying two subpopulations with different membrane docking behaviors. These subpopulations were regulated by clathrin and caveolin-1, and had opposing roles in the nuclear shuttling of SMAD4 and the subsequent transcriptional regulation of genes associated with cell migration. The notion that membrane-docking behaviors of downstream molecules could predict the cellular response to growth factors may revolutionize the way we view cell signaling.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000327400100005
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/46499]
专题	中国科学院化学研究所
通讯作者	Yang, Yong
作者单位	1.Houston Methodist Res Inst, Dept Nanomed, Houston, TX 77030 USA 2.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 5.Weill Cornell Med Coll, Dept Cell & Dev Biol, New York, NY 10065 USA 6.Weill Cornell Med Coll, Dept Med, New York, NY 10065 USA
推荐引用方式 GB/T 7714	Yang, Yong,Wolfram, Joy,Shen, Jianliang,et al. Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane[J]. FEBS LETTERS,2013,587(24):3912-3920.
APA	Yang, Yong.,Wolfram, Joy.,Shen, Jianliang.,Zhao, Yuliang.,Fang, Xiaohong.,...&Ferrari, Mauro.(2013).Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane.FEBS LETTERS,587(24),3912-3920.
MLA	Yang, Yong,et al."Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane".FEBS LETTERS 587.24(2013):3912-3920.