Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection

doi:10.1021/acsami.7b18275

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	Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection
	Liu, Shanshan 1,2; He, Ping 2; Hussain, Sameer 2; Lu, Huan 2; Zhou, Xin 2; Lv, Fengting 2; Liu, Libing 2; Dai, Zhihui 1; Wang, Shu 2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018-02-21
卷号	10 期号:7 页码:6618-6623
关键词	Photoelectrochemical Cytosensor Conjugated Polymers Turn-on Signal Skbr-3 Cell Sensitive Detection
ISSN号	1944-8244
DOI	10.1021/acsami.7b18275
英文摘要	In this work, a new photoelectrochemical (PEC) cytosensor was constructed by using cationic polyfluorene derivative, poly(9,9-bis(6'-(N,N,N,-trirnethylammonium)hexyl)fluorene-co-alt-1,4-phenylene)bromide (PFP) as the photoelectric-responsive material for sensitive cell detection. Positive-charged PFP with high photoelectric conversion efficiency can generate robust photocurrent under light illumination. In the PEC cytosensor, 3-phosphonopropionic acid was linked to the indium tin oxide electrode, followed by modification with antiepithelial-cell-adhesion-molecule (EpCAM) antibody via amide condensation reaction. Thus, target SKBR-3 cells with overexpressed EpCAM antigen could be captured onto the electrode via the specific antibody antigen interactions. Upon adding cationic PFP, a favorable electrostatic interaction between cationic PFP and negatively charged cell membrane led to a turn-on detection signal for target SKBR-3 cells. This new cytosensor not only exhibits good sensitivity because of the good photoelectric performance of conjugated polymers, but also offers decent selectivity to target cells by taking advantage of the specific antibody antigen recognition.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000426143900062
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/45687]
专题	中国科学院化学研究所
通讯作者	Dai, Zhihui; Wang, Shu
作者单位	1.Nanjing Normal Univ, Sch Chem & Mat Sci, Nanjing 210023, Jiangsu, Peoples R China 2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shanshan,He, Ping,Hussain, Sameer,et al. Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(7):6618-6623.
APA	Liu, Shanshan.,He, Ping.,Hussain, Sameer.,Lu, Huan.,Zhou, Xin.,...&Wang, Shu.(2018).Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection.ACS APPLIED MATERIALS & INTERFACES,10(7),6618-6623.
MLA	Liu, Shanshan,et al."Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection".ACS APPLIED MATERIALS & INTERFACES 10.7(2018):6618-6623.