Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium

doi:10.7503/cjcu20120906

CORC > 化学研究所 > 中国科学院化学研究所

	Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium
	Cha Dong-Mei 1; Han Zhao-Hui 1; Ma Tian 1; Li Bu-Hai 1; Liu Guo-Quan 2; Zhu Wei 3
刊名	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
	2013-04-10
卷号	34 期号:4 页码:760-762
关键词	Magnetic Microspheres Basic Fuchsin Bacterium Separation Mycobacterium
ISSN号	0251-0790
DOI	10.7503/cjcu20120906
英文摘要	Two kinds of methods were used to prepare basic fuchsin(BF) modified magnetic nanoparticles. The obtained BF modified nanoparticles (MNP-1 and MNP-2) were characterized using transmission electron microscope(TEM) and Fourier transformation infrared(FTIR) spectroscopy before used to adsorb mycobacterium from Staphylococcus aureus and Escherichia coli(E. coli). The effects of pH, adsorption time, concentration of bacteria, temperature, ionic strength and adsorbent dosage on the adsorption of bacterium were studied. The results show that both MNP-1 and MNP-2 have good selectivity for mycobacterium, and can be easily separated by external magnetic field. Under the optimized conditions, the highest adsorption rate for mycobacteria were 65% (MNP-1) and 85% (MNP-2), while only 20% of Staphylococcus aureus and 10% of E. coli can be adsorbed under the same conditions. The adsorption capacity of MNP-1 and MNP-2 for mycobacteria was 1. 48x10(10) and 3. 25x10(10) cfu/g, respectively.
语种	英语
出版者	HIGHER EDUCATION PRESS
WOS记录号	WOS:000317558400002
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/42192]
专题	中国科学院化学研究所
通讯作者	Li Bu-Hai
作者单位	1.South Cent Univ Nationalities, Coll Chem & Mat Sci, State Ethn Affairs Commiss, Key Lab Analyt Chem, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 3.South Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Cha Dong-Mei,Han Zhao-Hui,Ma Tian,et al. Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2013,34(4):760-762.
APA	Cha Dong-Mei,Han Zhao-Hui,Ma Tian,Li Bu-Hai,Liu Guo-Quan,&Zhu Wei.(2013).Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,34(4),760-762.
MLA	Cha Dong-Mei,et al."Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 34.4(2013):760-762.