Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers

doi:10.1007/s11434-012-5553-9

CORC > 化学研究所 > 中国科学院化学研究所

	Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers
	Xing BaoLing 1; Song JinZhao 2; Ge SuMei 1; Tang ZhengHua 1; Liu MengLu 1; Yang Qiong 2; Lu FengTing 2; Liu LiBing 2; Wang Shu 2
刊名	CHINESE SCIENCE BULLETIN
	2013-03-01
卷号	58 期号:8 页码:873-878
关键词	Conjugated Polymers Dna Mutation Kras Braf Sensors
ISSN号	1001-6538
DOI	10.1007/s11434-012-5553-9
英文摘要	The mutation detections of KRAS and BRAF genes are of significant importance to predict the responses to anti-cancer therapy and develop new drugs. In this paper, we developed a multi-step fluorescence resonance energy transfer (FRET) assay for multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers (CCP). The newly established detection system could detect as low as 2% mutant DNAs in DNA admixtures. By triggering the emission intensity change of CCP and the dyes labeled in the DNA, four possible statuses (three mutations and one wildtype) can be differentiated in one extension reaction. The detection efficiency of this new method in clinical molecular diagnosis was validated by determining KRAS and BRAF mutations of 51 formalin-fixed paraffin-embedded (FFPE) ovary tissue samples. Furthermore, the result of the CCP-based multi-step FRET assay can be directly visualized under UV light so that no expensive instruments and technical expertise are needed. Thus, the assay provides a sensitive, reliable, cost-effective and simple method for the detection of disease-related gene mutations.
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000316014600005
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/42088]
专题	中国科学院化学研究所
通讯作者	Yang Qiong
作者单位	1.Changzhou Wommen & Children Hlth Hosp, Dept Pathol, Changzhou 213003, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xing BaoLing,Song JinZhao,Ge SuMei,et al. Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers[J]. CHINESE SCIENCE BULLETIN,2013,58(8):873-878.
APA	Xing BaoLing.,Song JinZhao.,Ge SuMei.,Tang ZhengHua.,Liu MengLu.,...&Wang Shu.(2013).Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers.CHINESE SCIENCE BULLETIN,58(8),873-878.
MLA	Xing BaoLing,et al."Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers".CHINESE SCIENCE BULLETIN 58.8(2013):873-878.