Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata

doi:10.1021/acs.jafc.8b02708

CORC > 成都山地灾害与环境研究所 > 中国科学院水利部成都山地灾害与环境研究所

	Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata
	Fang Xianping 1,2; Xin Ya 3; Sheng Zheliang 4; Liu Hui 3; Jiang Aili 2; Wang Fang 5; Yang Jian 1; Xi Xiaojun 2; Zha Qian 2; Zhang Liqing 2
刊名	Journal of Agricultural and Food Chemistry
	2018
卷号	66 期号:50 页码:13310-13320
关键词	Amino acids Fruits Metabolism Molecular biology Plants (botany)
ISSN号	0021-8561
DOI	10.1021/acs.jafc.8b02708
产权排序	4
文献子类	Article
英文摘要	The various post-translational modifications (PTMs) of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines (Suks) in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen-stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition. Copyright © 2018 American Chemical Society.
电子版国际标准刊号	1520-5118
语种	英语
内容类型	期刊论文
源URL	[http://ir.imde.ac.cn/handle/131551/26621]
专题	中国科学院水利部成都山地灾害与环境研究所
通讯作者	Yan Chengqi
作者单位	1.Institute of Plant Virology, Ningbo University, Ningbo;315211, China; 2.Institute of Forestry and Pomology, Shanghai Academy of Agricultural Sciences, Shanghai;201403, China; 3.Hangzhou Academy of Agricultural Sciences, Hangzhou;310024, China; 4.Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu;610041, China; 5.Institute of Biotechnology, Ningbo Academy of Agricultural Sciences, Ningbo;315040, China; 6.College of Plant Protection, Hunan Agricultural University, Changsha;410128, China
推荐引用方式 GB/T 7714	Fang Xianping,Xin Ya,Sheng Zheliang,et al. Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata[J]. Journal of Agricultural and Food Chemistry,2018,66(50):13310-13320.
APA	Fang Xianping.,Xin Ya.,Sheng Zheliang.,Liu Hui.,Jiang Aili.,...&Chen Jianping.(2018).Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata.Journal of Agricultural and Food Chemistry,66(50),13310-13320.
MLA	Fang Xianping,et al."Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata".Journal of Agricultural and Food Chemistry 66.50(2018):13310-13320.