SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

	SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
	Chi, Xiaofeng ; Xing, Yuxiu ; Xiao, Yuancan ; Dong, Qi ; Hu, Fengzu
刊名	QUIMICA NOVA ; Chi, XF; Xing, YX; Xiao, YC; Dong, Q; Hu, FZ.SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY,QUIMICA NOVA,2014,37(9):1465
	2014
英文摘要	An effective method for the rapid separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was successfully established. In the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for HSCCC separation. A one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-O-glucoside, 42.0 mg of pieceid-2 ''-O-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these compounds were identified by nuclear magnetic resonance (NMR) spectroscopy.; An effective method for the rapid separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was successfully established. In the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for HSCCC separation. A one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-O-glucoside, 42.0 mg of pieceid-2 ''-O-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these compounds were identified by nuclear magnetic resonance (NMR) spectroscopy.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/4264]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chi, Xiaofeng,Xing, Yuxiu,Xiao, Yuancan,et al. SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY[J]. QUIMICA NOVA, Chi, XF; Xing, YX; Xiao, YC; Dong, Q; Hu, FZ.SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY,QUIMICA NOVA,2014,37(9):1465,2014.
APA	Chi, Xiaofeng,Xing, Yuxiu,Xiao, Yuancan,Dong, Qi,&Hu, Fengzu.(2014).SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY.QUIMICA NOVA.
MLA	Chi, Xiaofeng,et al."SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY".QUIMICA NOVA (2014).