Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis

	Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis
	LU Ting ; Edward P C Lai ; Jorn C C Yu ; HU Fengzu
刊名	食品科学 ; LU Ting,Edward P C Lai,Jorn C C Yu,HU Fengzu.Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis.食品科学,2006,27(12):582-587
	2006
英文摘要	A capillary electrophoresis (CE) method has been developed for the determination of six bioactive flavonoids that are commonly found in health foods: hesperidin, hyperin, isorhamnetin, kaempferol, quercetin and rutin. The effects of several parameters, such as pH, buffer concentration, separation voltage and UV detector wavelength, were investigated to find the optimal conditions. Using a HbBCh-NaiB-iO? buffer (pH 9.2), the analytes can be separated within 8 min. The relative standard deviations of migration times in eight injections were between 0.77% and 0.93%, and those of the peak areas ranged from 3.8% to 8.6%. A high reproducibility and excellent linearity was observed over two orders of magnitude, with detection limits (S/N = 3) ranging from 0.34ug ml to 2.9ug ml for all the six analytes. Recoveries ranged from 80.4 % to 113.9 %. The new method is simple, reproducible and sensitive. No solid phase extraction for sample pretreatment is necessary. Analysis results are accurate in application to bee pollens.; A capillary electrophoresis (CE) method has been developed for the determination of six bioactive flavonoids that are commonly found in health foods: hesperidin, hyperin, isorhamnetin, kaempferol, quercetin and rutin. The effects of several parameters, such as pH, buffer concentration, separation voltage and UV detector wavelength, were investigated to find the optimal conditions. Using a HbBCh-NaiB-iO? buffer (pH 9.2), the analytes can be separated within 8 min. The relative standard deviations of migration times in eight injections were between 0.77% and 0.93%, and those of the peak areas ranged from 3.8% to 8.6%. A high reproducibility and excellent linearity was observed over two orders of magnitude, with detection limits (S/N = 3) ranging from 0.34ug ml to 2.9ug ml for all the six analytes. Recoveries ranged from 80.4 % to 113.9 %. The new method is simple, reproducible and sensitive. No solid phase extraction for sample pretreatment is necessary. Analysis results are accurate in application to bee pollens.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/306]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	LU Ting,Edward P C Lai,Jorn C C Yu,et al. Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis[J]. 食品科学, LU Ting,Edward P C Lai,Jorn C C Yu,HU Fengzu.Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis.食品科学,2006,27(12):582-587,2006.
APA	LU Ting,Edward P C Lai,Jorn C C Yu,&HU Fengzu.(2006).Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis.食品科学.
MLA	LU Ting,et al."Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis".食品科学 (2006).